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FITC Mouse Anti-Human CD19 (Leu™-12)
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B4; B-lymphocyte antigen CD19; Leu-12; Leu12
Mouse BALB/c IgG1, κ
Human Chronic Lymphocytic Leukemia (CLL) Cells
Flow cytometry
25 μg/mL
20 μL
II B43
Phosphate buffered saline with gelatin and 0.1% sodium azide.


The monoclonal antibody is supplied as 50 μg purified immunoglobulin in 2.0 mL (25 μg/mL) of phosphate-buffered saline (PBS). The FITC conjugate is supplied as 50 μg in 2.0 mL (25 μg/mL). The PE conjugate is supplied as 25 μg/mL in 1.0 mL. The PerCP conjugate is supplied as 12.5 μg/mL in 2.0 mL (6.25 μg/mL). PBS contains gelatin and 0.1% sodium azide. Vials should be stored at 2° to 8°C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.

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The CD19 antibody, clone 4G7, is derived from hybridization of P3-X63-Ag8.653 mouse cells with spleen cells from BALB/c mice immunized with human chronic lymphocytic leukemia (CLL) cells.

The CD19 (Leu-12) antibody recognizes a human B-lymphocyte antigen, with a molecular weight of 90 kilodaltons (kDa).

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Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Blue 488 nm
494 nm
518 nm
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研发参考 (8)

  1. Bloomfield C, Gajl-Peczalska K, Frizzera G, Kersey J, Goldman A. Clinical utility of surface markers combined with Lukes-Collins histologic classification in adult lymphoma. N Eng J Med. 1979; 301:512. (Biology).
  2. Dörken B, Möller P, Pezzutto A, Schwartz-Albiez R, Moldenhauer G. Knapp W, Dörken B, Gilks WR, et al, ed. Leucocyte Typing IV: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1989:34-36.
  3. Foucar K, Goeken JA. Clinical application of immunologic techniques to the diagnosis of lymphoproliferative and immunodeficiency disorders. Lab Med. 1982; 13:403-413. (Biology).
  4. Loken MR, Shah VO, Dattilio KL, Civin CI. Flow cytometric analysis of human bone marrow. II. Normal B lymphocyte development. Blood. 1987; 70(5):1316-1324. (Biology). 查看参考
  5. Meeker TC, Miller RA, Link MP, Bindl J, Warnke R, Levy R. A unique human B lymphocyte antigen defined by a monoclonal antibody.. Hybridoma. 1984; 3(4):305-20. (Biology). 查看参考
  6. Moldenhauer G, Dörken B, Schwartz R, Pezzutto A, Knops J, Hammerling GJ. Analysis of ten B lymphocyte-specific workshop monoclonal antibodies. In: Reinherz EL, Haynes BF, Nadler LM, Bernstein ID, ed. Leukocyte Typing II: Human B Lymphocytes. New York: Springer-Verlag; 1986:61-67.
  7. Reichert T, DeBruyere M, Deneys V, et al. Lymphocyte subset reference ranges in adult Caucasians. Clin Immunol Immunopathol. 1991; 60(2):190-208. (Biology). 查看参考
  8. Warnke RA, Link MD. Identification and significance of cell markers in leukemia and lymphoma. Ann Rev Med. 1983; 34:117. (Biology).
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347543 Rev. 1

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