APC Mouse Anti-Human CD25

BD™ APC Mouse Anti-Human CD25

Name: APC Mouse Anti-Human CD25
Brand: BD™
SKU: 340939

克隆 2A3

(RUO (GMP))

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商品详情

Brand: BD™

Alternative Name: IL-2R; IL2RA; IL-2Rα; TCGFR; TAC antigen; p55

Reactivity: Human

Isotype: Mouse BALB/c IgG1, κ

Immunogen: Human Phytohemagglutinin-activated T Cells

Application: Flow cytometry

Concentration: 12 μg/mL

Vol. Per Test: 5 μL

Workshop Number: III A769,T153; IV A8

Entrez Gene ID: 3559

RRID: AB_400551

Storage Buffer: Phosphate buffered saline with gelatin and 0.1% sodium azide.

Regulatory Status: RUO (GMP)

准备和存储

The monoclonal antibody is supplied as 10 µg puriﬁed immunoglobulin in 2.0 mL (5 µg/mL) of PBS. The FITC conjugate is supplied as 20 µg in 2.0 mL (10 µg/mL) of PBS. The PE conjugate is supplied as 3 µg in 1.0 mL (3 µg/mL) of PBS. The PE-Cy7 conjugate is supplied as 6.25 µg in 0.5 mL (12.5 µg/mL) of PBS. The APC conjugate is supplied as 6 µg in 0.5 mL (12 µg/mL) of PBS. PBS contains gelatin and 0.1% sodium azide.

Store vials at 2° to 8°C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.

340939 Rev. 1

抗体详情

2A3

The CD25 antibody, clone 2A3. is derived from the hybridization of NS-1 mouse myeloma cells with spleen cells isolated from BALB/c mice immunized with PHA-activated human T lymphocytes.

The CD25 antibody recognizes a 55-kilodalton (kDa) type I integral membrane glycoprotein. The CD25 antigen is also known as the human low-affinity interleukin-2 receptor α chain (IL-2Rα) and the Tac antigen.

340939 Rev. 1

格式详情

APC

Allophycocyanin (APC), is part of the BD family of phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 651 nm and an emission maximum (Em Max) at 660 nm. APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

格式: APC

激发源: Red 627-640 nm

最多激发源: 651 nm

最多发射源: 660 nm

340939 Rev.1

报价单和参考

研发参考 (13)

Bach JF. Regulatory T cells under scrutiny. Nat Rev Immunol. 2003; 3(3):189-198. (Biology). 查看参考
Greene WC, Leonard WJ. The human interleukin-2 receptor. Annu Rev Immunol. 1986; 4:69-95. (Biology). 查看参考
Jackson AL, Matsumoto H, Janszen M, Maino V, Blidy A, Shye S. Restricted expression of p55 interleukin 2 receptor (CD25) on normal T cells. Clin Immunol Immunopathol. 1990; 54(1):126-133. (Biology). 查看参考
Lando Z, Sarin P, Megson M, et al. Association of human T-cell leukaemia/lymphoma virus with the Tac antigen marker for the human T-cell growth factor receptor. Nature. 1983; 305(5936):733-736. (Biology). 查看参考
Leonard WJ, Depper JM, Uchiyama T, Smith KA, Waldmann TA, Greene WC. A monoclonal antibody that appears to recognize the receptor for human T-cell growth factor; partial characterization of the receptor. Nature. 1982; 300(5889):267-269. (Biology). 查看参考
Ng WF, Duggan PJ, Ponchel F, et al. Human CD4(+)CD25(+) cells: a naturally occurring population of regulatory T cells. Blood. 2001; 98(9):2736-2744. (Biology). 查看参考
Rambaldi A, Young DC, Herrmann F, Cannistra SA, Griffin JD. Interferon-gamma induces expression of the interleukin 2 receptor gene in human monocytes. Eur J Immunol. 1987; 17(1):153-156. (Biology). 查看参考
Robb RJ, Greene WC, Rusk CM. Low and high affinity cellular receptors for interleukin 2. Implications for the level of Tac antigen. J Exp Med. 1984; 160(4):1126-1146. (Biology). 查看参考
Schwarting R, Stein H. Knapp W, Dörken B, Gilks WR, et al, ed. Leucocyte Typing IV: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1989:455-460.
Sereti I, Martinez-Wilson H, Metcalf JA, et al. Long-term effects of intermittent interleukin 2 therapy in patients with HIV infection: characterization of a novel subset of CD4+CD25+ T cells. Blood. 2002; 100:2159-2167. (Biology).
Siegel JP, Sharon M, Smith PL, Leonard WJ. The IL-2 receptor beta chain (p70): role in mediating signals for LAK, NK, and proliferative activities. Science. 1987; 238(4823):75-78. (Biology). 查看参考
Teshigawara K, Wang HM, Kato K, Smith KA. Interleukin 2 high-affinity receptor expression requires two distinct binding proteins. J Exp Med. 1987; 165(1):223-238. (Biology). 查看参考
Urdal DL, March CJ, Gillis S, Larsen A, Dower SK. Purification and chemical characterization of the receptor for interleukin 2 from activated human T lymphocytes and from a human T-cell lymphoma cell line. Proc Natl Acad Sci U S A. 1984; 81(20):6481-6485. (Biology). 查看参考

查看所有文件 (13)

340939 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Although not required, these products are manufactured in accordance with Good Manufacturing Practices.