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Anti-Human CD86 PE/CD209 PerCP-Cy™5.5/CD83 APC

Anti-Human CD86 PE/CD209 PerCP-Cy™5.5/CD83 APC

(RUO (GMP))
商品详情
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BD™
Human
Flow cytometry
RUO (GMP)
Phosphate buffered saline with BSA, beta-lactoglobulin, and 0.1% sodium azide.


描述

CD86, clone FUN-1, is derived from hybridization of Sp-2 x HBL-1.

CD209, clone DCN46, is cloned from a placental library.

CD83, clone HB15e, is derived from Balb/c x NS-1.

The CD86 antibody recognizes a 75-kilodalton (kDa) cell-surface protein, a second ligand for CD28 and CTLA-4 expressed on T cells.

The CD209 antibody recognizes a dendritic cell–specific ICAM-3–grabbing nonintegrin (DC-SIGN). The CD209 antigen is a type II membrane protein of approximately 44 kDa, with a mannose-binding, C-type lectin domain exclusively expressed by dendritic cells, both in vitro–generated MDDCs and dendritic cells in several tissues, including mucosa and lymph nodes.

The CD83 antibody reacts with a 45-kDa transmembrane protein member of the Ig superfamily and is composed of a single V-type Ig extracellular domain with a Cterminal cytoplasmic tail.

准备和存储

Store vials at 2°C–8°C. Conjugated forms should not be frozen. Protect from exposure to light. Each reagent is stable until the expiration date shown on the bottle label when stored as directed.

334098 Rev. 1
组件
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描述 克隆 同型对照 EntrezGene ID
CD83 APC HB15e IgG1, κ 9308,942
CD86 (B7-2) PE 2331 (FUN-1) IgG1, κ N/A
CD209 (DC-SIGN) PerCP-Cy5.5 DCN46 IgG2b, κ 9308,942
334098 Rev. 1
报价单和参考
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研发参考 (33)

  1. Alvarez CP, Lasala F, Carrillo J, Muñiz O, Corbí AL, Delgado R. C-type lectins DC-SIGN and L-SIGN mediate cellular entry by ebola virus in cis and in trans. J Virology. 2002; 76:6841-6844. (Biology).
  2. Azuma M, Ito D, Yagita H, et al. B70 antigen is a second ligand for CTLA-4 and CD28.. Nature. 1993; 366(6450):76-9. (Biology). 查看参考
  3. Baribaud F, Pöhlmann S, Leslie G, Mortari F, Doms RW. Quantitative expression and virus transmission analysis of DC-SIGN on monocyte-derived dendritic cells. J Virol. 2002; 76(18):9135-9142. (Biology). 查看参考
  4. Centers for Disease Control. Update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in healthcare settings. MMWR. 1988; 37:377-388. (Biology).
  5. Clinical and Laboratory Standards Institute. 2005. (Biology).
  6. Dhodapkar MV, Steinman RM, Sapp M, et al. Rapid generation of broad T-cell immunity in humans after a single injection of mature dendritic cells. J Clin Invest. 1999; 104:173-180. (Biology).
  7. Engel P, Gribben JG, Freeman GJ, et al. The B7-2 (B70) costimulatory molecule expressed by monocytes and activated B lymphocytes is the CD86 differentiation antigen. Blood. 1994; 84(5):1402-1407. (Biology). 查看参考
  8. Engel P, Wagner N, Tedder TF. Schlossman SF, Boumsell L, Gilks W, et al, ed. Leucocyte Typing V: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1995:703-705.
  9. Engering A, van Vliet SJ, Geijtenbeek TBH, van Kooyk Y. Subset of DC-SIGN+ dendritic cells in human blood transmits HIV-1 to T lymphocytes. Blood. 2002; 100:1780-1786. (Biology).
  10. Geijtenbeek TBH, Krooshoop DJEB, Bleijs DA, et al. DC-SIGN–ICAM-2 interaction mediates dendritic cell trafficking. Nature. 2000; 1:353-357. (Biology).
  11. Geijtenbeek TBH, Kwon DS, Torensma R, et al. DC-SIGN, a dendritic cell-specific HIV-1 -binding protein that enhances trans-infection of T cells. Cell. 2000; 100(5):587-597. (Biology). 查看参考
  12. Geijtenbeek TBH, Torensma R, van Vliet SJ, et al. Identification of DC-SIGN, a novel dendritic cell-specific ICAM-3 receptor that supports primary immune responses. Cell. 2000; 100(5):575-585. (Biology). 查看参考
  13. Geijtenbeek TBH, van Duijnhoven GCF, van Vliet SJ, et al. Identification of different binding sites in the dendritic cell-specific receptor DC-SIGN for intercellular adhesion molecule 3 and HIV-1. J Biol Chem. 2002; 277:11314-11320. (Biology).
  14. Geijtenbeek TBH, van Vliet SJ, Koppel EA, et al. Mycobacteria target DC-SIGN to suppress dendritic cell function. J Exp Med. 2003; 197:44029. (Biology).
  15. Hart DN. Dendritic cells: unique leukocyte populations which control the primary immune response. Blood. 1997; 90(9):3245-3287. (Biology). 查看参考
  16. Kruse M, Meinl E, Henning G, et al. Signaling lymphocytic activation molecule is expressed on mature CD83+ dendritic cells and is up-regulated by IL-1β. J Immunol. 2001; 167:1989-1995. (Biology).
  17. MacDonald AS, Straw AD, Bauman B, Pearce EJ. CD8- dendritic cell activation status plays an integral role in influencing Th2 response development. J Immunol. 2001; 167:1982-1988. (Biology).
  18. Marovich MA, Mascola JR, Eller MA, et al. Preparation of clinical-grade recombinant canarypox–human immunodeficiency virus vaccine–loaded human dendritic cells. J Infec Dis. 2002; 185:1242-1252. (Biology).
  19. Matsui M, Moriya O, Abdel-Aziz N, Matsuura Y, Miyamura T, Akatsuka T. Induction of hepatitis C virus-specific cytotoxic T lymphocytes in mice by immunization with dendritic cells transduced with replication-defective recombinant adenovirus. Vaccine 21. 2002; 211-220. (Biology).
  20. Nozawa Y, Wachi E, Tominaga K, Abe M, Wakasa H. A novel monoclonal antibody (FUN-1) identifies an activation antigen in cells of the B-cell lineage and Reed-Sternberg cells. J Pathol. 1993; 169(3):309-315. (Biology). 查看参考
  21. Osugi Y, Vuckovic S, Hart DNJ. Myeloid blood CD11c+ dendritic cells and monocyte-derived dendritic cells differ in their ability to stimulate T lymphocytes. Blood. 2002; 100:2858-2866. (Biology).
  22. Reddy A, Sapp M, Feldman M, Subklewe M, Bhardwaj N. A monocyte conditioned medium is more effective than defined cytokines in mediating the terminal maturation of human dendritic cells. Blood. 1997; 90:3640-3646. (Biology).
  23. Relloso M, Puig-Kröger A, Pello OM, et al. DC-SIGN (CD209) expression is IL-4 dependent and is negatively regulated by IFN, TGF-β, and anti-inflammatory agents. J Immunol. 2002; 168:2634-2643. (Biology).
  24. Steinman RM. DC-SIGN: a guide to some mysteries of dendritic cells. Cell. 2000; 100:491-494. (Biology).
  25. Subauste CS, Wessendarp M. Human dendritic cells discriminate between viable and killed toxoplasma gondii tachyzoites: dendritic cell activation after infection with viable parasites results in CD28 and CD40 ligand signaling that controls IL-12-dependent and -independent T cell production of IFN-γ. J Immunol. 2000; 165:1498-1505. (Biology).
  26. Summers KL, Daniel PB, O'Donnell JL, Hart DN. Dendritic cells in synovial fluid of chronic inflammatory arthritis lack CD80 surface expression. Clin Exp Immunol. 1995; 100(1):81-89. (Biology). 查看参考
  27. Tailleux L, Schwartz O, Herrmann J-L, et al. DC-SIGN is the major mycobacterium tuberculosis receptor on human dendritic cells. J Exp Med. 2003; 197:121-127. (Biology).
  28. Tassaneetrithep B, Burgess TH, Granelli-Piperno A, et al. DC-SIGN (CD209) mediates dengue virus infection of human dendritic cells. J Exp Med. 2003; 197(7):823-829. (Biology). 查看参考
  29. Thurner B, Röder C, Dieckmann D, et al. Generation of large numbers of fully mature and stable dendritic cells from leukapheresis products for clinical application. J Immmol Meth. 1999; 223:43845. (Biology).
  30. Weissman D, Li Y, Ananworanich J, et al. Three populations of cells with dendritic morphology exist in peripheral blood, only one of which is infectable with human immunodeficiency virus type 1. Proc Natl Acad Sci USA. 1995; 92:826-830. (Biology).
  31. Zhou LJ, Schwarting R, Smith HM, Tedder TF. A novel cell-surface molecule expressed by human interdigitating reticulum cells, Langerhans cells, and activated lymphocytes is a new member of the Ig superfamily. J Immunol. 1992; 149(2):735-742. (Biology). 查看参考
  32. Zhou LJ, Tedder TF. CD14+ blood monocytes can differentiate into functionally mature CD83+ dendritic cells. Proc Natl Acad Sci U S A. 1996; 93(6):2588-2592. (Biology). 查看参考
  33. Zhou LJ, Tedder TF. Human blood dendritic cells selectively express CD83, a member of the immunoglobulin superfamily. J Immunol. 1995; 154(8):3821-3835. (Biology). 查看参考
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334098 Rev. 1

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