Mouse IgG1 PerCP-Cy™5.5

BD™ Mouse IgG1 PerCP-Cy™5.5

Name: Mouse IgG1 PerCP-Cy™5.5
Brand: BD™
SKU: 347212

克隆 X40

(ASR)

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科学资源

商品详情

Brand: BD™

Alternative Name: IgG1, kappa Isotype Control (Anti-KLH)

Isotype: Mouse BALB/c IgG1, κ

Immunogen: Keyhole limpet hemocyanin (KLH)

Application: Flow cytometry

Concentration: 25 µg/mL

Vol. Per Test: 20 μL

Storage Buffer: Phosphate buffered saline with gelatin and 0.1% sodium azide.

Regulatory Status: ASR

准备和存储

The antibody was conjugated with PerCP-Cy5.5 under optimum conditions, and unconjugated antibody and free PerCP-Cy5.5 were removed. Storage of PerCP-Cy5.5 conjugates in unoptimized diluent is not recommended and may result in loss of signal intensity. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Store vials at 2°C–8°C. Conjugated forms should not be frozen. Protect from exposure to light. Each reagent is stable until the expiration date shown on the bottle label when stored as directed.

商品通知

Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Cy is a trademark of Amersham Biosciences Limited. This conjugated product is sold under license to the following patents: US Patent Nos. 4,876,190; 5,268,486; 5,486,616; 5,569,587; 5,569,766; and 5,627,027.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

347212 Rev. 1

抗体详情

X40

The Mouse IgG₁ antibody, clone X40, is derived from the hybridization of Sp2/0-Ag 14 mouse myeloma cells with spleen cells from BALB/c mice immunized with KLH.

The antibodies react specifically with keyhole limpet hemocyanin (KLH), an antigen not expressed on human cells or human cell lines.

347212 Rev. 1

格式详情

PerCP-Cy5.5

PerCP-Cy5.5 dye is part of the BD blue family of dyes. This tandem fluorochrome is comprised of a fluorescent protein complex (PerCP) with an excitation maximum (Ex Max) of 482 nm and an acceptor dye with an emission maximum (Em Max) at 676 nm. PerCP-Cy5 is designed to be excited by the blue laser (488-nm) and detected using an optical filter centered near 680 nm (e.g., a 695/40 nm bandpass filter). The donor dye can be partially excited by the Violet (405-nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

格式: PerCP-Cy5.5

激发源: Blue 488 nm

最多激发源: 482 nm

最多发射源: 676 nm

347212 Rev.1

报价单和参考

研发参考 (2)

Centers for Disease Control. Update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in healthcare settings. MMWR. 1988; 37:377-388. (Biology).
Clinical and Laboratory Standards Institute. 2005. (Biology).

347212 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.