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SELP; PSEL; P-selectin; PADGEM; LECAM3; GMP-140; GRMP; GMP140
Mouse BALB/c IgG1, κ
Activated Human Platelets
Flow cytometry
1.5 μg/mL
20 μL
V S058

654976 Rev. 1
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The CD62P antibody, clone AC1.2, is derived from the hybridization of NS-1 mouse myeloma cells with spleen cells isolated from BALB/c mice immunized with activated platelets.

The CD62P antibody recognizes a molecule known as platelet activation-dependent granule-external membrane (PADGEM) protein or granule membrane protein (GMP-140), which is a 140-kilodalton (kDa) single-chain polypeptide. The CD62P antigen is a member of the selectin family of adhesion molecules and mediates the adhesion of activated platelets to neutrophils and monocytes in hemostasis.

654976 Rev. 1
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R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
654976 Rev.1
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研发参考 (12)

  1. Ault KA, Rinder HM, Mitchell JG, Rinder CS, Lambrew CT, Hillman RS. Correlated measurement of platelet release and aggregation in whole blood. Cytometry. 1989; 10:448-455. (Biology).
  2. Berman CL, Yeo EL, Wencel-Drake JD, Furie BC, Ginsberg MH, Furie B. A platelet α-granule membrane protein that is associated with the plasma membrane after activation. J Clin Invest. 1986; 78:130-137. (Biology).
  3. Bonfanti R, Furie BC, Furie B, Wagner DD. PADGEM (GMP-140) is a component of Weibel-Palade bodies of human endothelial cells. Blood. 1989; 73:1109-1112. (Biology).
  4. Centers for Disease Control. Update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in healthcare settings. MMWR. 1988; 37:377-388. (Biology).
  5. Clinical and Laboratory Standards Institute. 2005. (Biology).
  6. Diacovo T, Springer TA. CD62L (L-selectin) cluster report. In: Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995:1503-1504.
  7. Hamburger SA, McEver RP. GMP-140 mediates adhesion of stimulated platelets to neutrophils. Blood. 1990; 75:550-554. (Biology).
  8. Larsen E, Celi A, Gilbert GE, et al. PADGEM protein: a receptor that mediates the interaction of activated platelets with neutrophils and monocytes.. Cell. 1989; 59(2):305-12. (Biology). 查看参考
  9. Metzelaar MJ, Sixma JJ, Nieuwenhuis HK. Detection of platelet activation using activation-specific monoclonal antibodies. Blood Cells. 1990; 16:85-96. (Biology).
  10. Modderman PW. Knapp W, Dörken B, Gilks WR, et al, ed. Leucocyte Typing IV: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1989:1038-1039.
  11. Parmentier S, Kaplan C, Catimel B, McGregor JL. New families of adhesion molecules play a vital role in platelet functions.. Immunol Today. 1990; 11(7):225-7. (Biology). 查看参考
  12. Stenberg PE, McEver RP, Shuman MA, Jacques YV, Bainton DF. A platelet α-granule membrane protein (GMP-140) is expressed on the plasma membrane after activation. J Cell Biol. 1985; 101:880-886. (Biology).
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654976 Rev. 1

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