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Mouse BALB/c x DBA/2, also known as CD2F1 or CDF1 IgG1, κ
Human polymorphonuclear leukocytes
IV N409; V MR5, NK80
Phosphate buffered saline with gelatin and 0.1% sodium azide.
Store vials at 2°C to 8°C. Conjugated forms should not be frozen and should be protected from exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.
The CD16 antibody, clone 3G8, is derived from the fusion of CD2F1 mouse spleen cells with P3U1 myeloma cells.
The CD16 antibodies recognize a 50 to 65-kilodalton (kDa) antigen present on human NK lymphocytes that is the IgG Fc receptor III.
338433 Rev. 1
PerCP-Cy5.5 dye is part of the BD blue family of dyes. This tandem fluorochrome is comprised of a fluorescent protein complex (PerCP) with an excitation maximum (Ex Max) of 482 nm and an acceptor dye with an emission maximum (Em Max) at 676 nm. PerCP-Cy5 is designed to be excited by the blue laser (488-nm) and detected using an optical filter centered near 680 nm (e.g., a 695/40 nm bandpass filter). The donor dye can be partially excited by the Violet (405-nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Blue 488 nm
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