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Anti-Human IFN-γ APC
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Mouse IgG2b
Recombinant human IFN-γ
Flow cytometry
12 μg/mL
5 μL

655933 Rev. 1
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The anti-Hu–IFN-γ antibody, clone 25723.11, is derived from the hybridization of mouse P3X-63-Ag8.653 myeloma cells with lymph node cells from BALB/c mice immunized with recombinant human IFN-γ.

The anti-human interferon-γ (anti-Hu–IFN-γ) antibody recognizes a 20- to 25-kilodalton (kDa) glycoprotein, which is a type II interferon. It is also known as immune interferon.

655933 Rev. 1
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Allophycocyanin (APC), is part of the BD family of phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 651 nm and an emission maximum (Em Max) at 660 nm. APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Red 627-640 nm
651 nm
660 nm
655933 Rev.1
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研发参考 (16)

  1. Aggarwal B, Puri R. Human Cytokines: Their Role in Disease and Therapy. Cambridge, MA: Blackwell Science; 1995:3-24.
  2. Buhtoiarov IN, Lum H, Berke G, Paulnock DM, Sondel PM, Rakhmilevich AL. CD40 ligation activates murine macrophages via an IFN-γ-dependent mechanism resulting in tumor cell destruction in vitro. J Immunol. 2005; 174:6013-6022. (Biology).
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  5. ElGhazali GEB, Paulie S, Andersson G, et al. Number of interleukin-4– and interferon- γ –secreting human T cells reactive with tetanus toxoid and the mycobacterial antigen PPD or phytohemagglutindistinct response profiles depending on the type of antigen used for activation. Eur J Immunol. 1993; 23:2740-2745. (Biology).
  6. Hardy KJ, Sawada T. Human γ interferon strongly upregulates its own gene expression in peripheral blood lymphocytes. J Exp Med. 1989; 170:1021-1026. (Biology).
  7. Jarasch N, Martin U, Kamphausen E, Zell R, Wutzler P, Henke A. Interferon-γ-induced activation of nitric oxide-mediated antiviral activity of macrophages caused by a recombinant coxsackievirus B3. Viral Immunol. 2005; 18:355-364. (Biology).
  8. Johnson HM, Bazer FW, Szente BE, Jarpe MA. How interferons fight disease. Scientific American. 1994; May:68-75. (Biology).
  9. Murray HW, Spitalny GL, Nathan CF. Activation of mouse peritoneal macrophages in vitro and in vivo by interferon-γ. J Immunol. 1990; 13:411-421. (Biology).
  10. Murray HW. γ interferon, cytokine-induced macrophage activation, and antimicrobial host defense. In vitro, in animal models, and in humans. Diagn Microbiol Infect Dis. 1990; 13:411-421. (Biology).
  11. Openshaw P, Murphy EE, Hosken NA, et al. Heterogeneity of intracellular cytokine synthesis at the single-cell level in polarized T helper 1 and T helper 2 populations. J Exp Med. 1995; 182(5):1357-1367. (Biology). 查看参考
  12. Paliard X, Malefijt RDW, Yssel H, et al. Simultaneous production of IL-2, IL-4, and IFN-γ by activated human CD4+ and CD8+ T cell clones. J Immunol. 1988; 141:849-855. (Biology).
  13. Powrie F, Coffman RL. Cytokine regulation of T-cell function: potential for therapeutic intervention. Immunol Today. 1993; 14:270-274. (Biology).
  14. Protection of Laboratory Workers from Occupationally Acquired Infections. Approved Guideline. 2005; M29-A3. (Biology).
  15. Romagnani S. Human TH1 and TH2 subsets: regulation of differentiation and role in protection and immunopathology. Int Arch Allergy Immunol. 1992; 98:279-285. (Biology).
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655933 Rev. 1

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