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β2-Microglobulin PerCP-Cy™5.5
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Mouse IgM, κ
Flow cytometry
25 μg/mL
20 μL

655435 Rev. 1
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The β-Microglobulin antibody, clone TÜ99, is derived from the hybridization of P3-NS1/1-Ag4-1 mouse myeloma cells with spleen cells isolated from BALB/c mice immunized with a human uncloned primed lymphocyte typing (PLT) cell line.

The β-Microglobulin antibody specifically recognizes β-microglobulin, a 12-kilodalton (kDa) protein with homology to the constant region of immunoglobulin molecules.

655435 Rev. 1
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PerCP-Cy5.5 dye is part of the BD blue family of dyes. This tandem fluorochrome is comprised of a fluorescent protein complex (PerCP) with an excitation maximum (Ex Max) of 482 nm and an acceptor dye with an emission maximum (Em Max) at 676 nm. PerCP-Cy5 is designed to be excited by the blue laser (488-nm) and detected using an optical filter centered near 680 nm (e.g., a 695/40 nm bandpass filter). The donor dye can be partially excited by the Violet (405-nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Blue 488 nm
482 nm
676 nm
655435 Rev.1
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研发参考 (10)

  1. Bix M, Coles M, Raulet D. Positive selection of Vβ8+CD4-8- thymocytes by class I molecules expressed by hematopoietic cells. J Exp Med. 1993; 178:901-908. (Biology).
  2. Centers for Disease Control. Update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in healthcare settings. MMWR. 1988; 37:377-388. (Biology).
  3. Clinical and Laboratory Standards Institute. 2005. (Biology).
  4. Grey HM, Kubo RT, Colon SM, et al. The small subunit of HL-A antigens is β 2-microglobulin. J Exp Med. 1973; 138:1608-1612. (Biology).
  5. Koller BH, Marrack P, Kappler JW, Smithies O. Normal development of mice deficient in β2M, MHC class I proteins, and CD8+ T cells. Science. 1990; 248:1227-1230. (Biology).
  6. Martin D, Fauchet R, Müller C, et al. Expression of HLA-A and -B antigens on differentiating U-937 cells. Tissue Antigens. 1985; 25:235-246. (Biology).
  7. Peterson PA, Cunningham BA, Berggård I, Edelman GM. β2</sub-Microglobulin—a free immunoglobulin domain. Proc Natl Acad Sci USA. 1972; 69:1697-1701. (Biology).
  8. Peterson PA, Rask L, Lindblom JB. Highly purified papain-solubilized HL-A antigens contain β2-microglobulin. Proc Natl Acad Sci USA. 1974; 71:35-39. (Biology).
  9. Vitiello A, Potter TA, Sherman LA. The role of β 2-microglobulin in peptide binding by class I molecules. Science. 1990; 250:1423-1426. (Biology).
  10. Zijlstra M, Bix M, Simister NE, Loring JM, Raulet DH, Jaenisch R. β2-Microglobulin deficient mice lack CD4-8+ cytolytic T cells. Nature. 1990; 344:742-746. (Biology).
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655435 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.