Welcome to the Introduction to Flow Cytometry Web-Based Training.

System Requirements

This course is best viewed using Internet Explorer ® version 6 or later, Safari v3.2.1 or later, or Chrome 3.0.195 or later web browsers, and Adobe ® flash player 8.0 or later.

Course Objectives

  • Define key flow cytometry terms.
  • Describe and explain key flow cytometry principles.
  • Discuss the components and functions of the fluidics, optics, and electronics systems.
  • Explain how data is analyzed.
  • Describe the sorting process on flow cytometers.

Intro to Flow icon - Overview


  • Define the term flow cytometry.
  • Describe the types of samples suitable for a flow cytometer.
  • Explain the properties that a flow cytometer can measure.
  • Identify the three subsystems of a flow cytometer.

Intro to Flow icon - Fluidics


  • Describe the functions of the fluidics system.
  • Explain hydrodynamic focusing.
  • Describe how sample pressure affects flow rate and data resolution.

Intro to Flow icon - Optics


  • Describe the functions of the optics system.
  • Discuss the role of lasers in BD cytometers.
  • Explain how filters route light to appropriate detectors.

Intro to Flow icon - Electronics


  • Describe how the electronics system converts light signals into numerical data.
  • Explain how laser time delays ensure that signals from multiple lasers are attributed to the correct event.
  • Explain how and why thresholds are used.

Intro to Flow icon - Optical Measurement

Optical Measurements

  • Describe the factors that affect light scatter.
  • Explain how light scatter and fluorescence can be used to detect populations.
  • Describe the use of excitation and emission spectra.
  • Explain what compensation is and why it is necessary for multicolor analysis.

Intro to Flow icon - Data

Data Analysis

  • Describe how data is saved and displayed.
  • Explain the purpose of gating.
  • State examples of data analysis methods.

Intro to Flow icon - Sorting


  • Describe the purpose of sorting cells or particles.
  • Explain how particle size affects the nozzle size and sheath pressure selection.
  • Describe the steps in droplet sorting.

Course Evaluation: Provide us with feedback to improve the course.

BD FACSCalibur™, BD FACSCanto™ II, and BD FACSCount™ are for In Vitro Diagnostic use.

BD FACSAria™ III, BD FACSArray ™, BD Influx™, BD™ LSR II, and BD LSRFortessa™ are for Research Use Only. Not for use in diagnostic or therapeutic procedures.

Class I (1) Laser Products.