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Oligo Mouse Anti-Human Podoplanin

BD™ AbSeq Oligo Mouse Anti-Human Podoplanin

Clone LpMab-17

(RUO)
Product Details
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BD™ AbSeq
PDPN; T1-alpha (T1A); GP36; GP40; OTS8; T1A2; TI1A; T1A-2; AGGRUS; PA2.26
10630
2 µl
Mouse BALB/c IgG1, κ
Human (Tested in Development)
Single Cell 3' Sequencing (Qualified)
TTTATGAGTATTACGTCTGTTGCGATTGTTGGCGGT
AHS0192
Human PDPN Transfected Cell Line
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO
Mouse


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography and conjugated to BD® AbSeq oligonucleotide under optimal conditions.

Recommended Assay Procedures

Put all BD® AbSeq Reagents to be pooled into a Latch Rack for 500 µL Tubes (Thermo Fisher Scientific Cat. No. 4900). Arrange the tubes so that they can be easily uncapped and re-capped with an 8-Channel Screw Cap Tube Capper (Thermo Fisher Scientific Cat. No. 4105MAT) and the reagents aliquoted with a multi-channel pipette.

BD® AbSeq tubes should be centrifuged for ≥ 30 seconds at 400 × g to ensure removal of any content in the cap/tube threads prior to the first opening.

Product Notices

  1. This reagent has been pre-diluted for use at the recommended volume per test. Typical use is 2 µl for 1 × 10^6 cells in a 200-µl staining reaction.
  2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
  5. Illumina is a trademark of Illumina, Inc.
  6. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
  7. Please refer to bd.com/genomics-resources for technical protocols.
  8. For U.S. patents that may apply, see bd.com/patents.
940270 Rev. 2
Antibody Details
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LpMab-17

The LpMab-17 monoclonal antibody specifically recognizes human podoplanin which is encoded by PDPN. Podoplanin is a 38-44 kDa type I transmembrane glycoprotein. This heavily glycosylated mucin type protein is named for its expression on kidney glomerular epithelial cells known as podocytes. It is also expressed on a wide variety of cell types, including intestinal and thymic epithelial cells, alveolar type I cells, fibroblastic reticular cells, lymphatic endothelial cells, osteoblasts, and Th17 cells. Its expression is upregulated in some tumors. Podoplanin induces platelet aggregation via its three platelet aggregation-stimulating (PLAG) domains; it binds C-type lectin domain family 1 member B (Clec1B, also known as CLEC2); and it is involved in actin cytoskeleton organization and in cellular adhesion and migration. It also plays roles in organogenesis, vascular development, inflammatory diseases, tumorigenesis, and cancer cell motility and metastasis.

940270 Rev. 2
Format Details
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Antibody-Oligo
The antibody was conjugated to an oligonucleotide that contains an antibody clone-specific barcode (ABC) flanked by a poly-A tail on the 3' end and a PCR handle (PCR primer binding site) on the 5' end. The ABC for this antibody was designed to be used with other BD® AbSeq oligonucleotides conjugated to other antibodies. All AbSeq ABC sequences were selected in silico to be unique from human and mouse genomes, have low predicted secondary structure, and have high Hamming distance within the BD® AbSeq portfolio, to allow for sequencing error correction and unique mapping. The poly-A tail of the oligonucleotide allows the ABC to be captured by the BD Rhapsody™ system. The 5' PCR handle allows for efficient sequencing library generation for Illumina sequencing platforms.NOTE: The BD Rhapsody™ Single-Cell Analysis System must be used with the BD Rhapsody™ Express Instrument.
Antibody-Oligo
940270 Rev.2
Citations & References
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Development References (5)

  1. Astarita JL, Acton SE, Turley SJ. Podoplanin: emerging functions in development, the immune system, and cancer.. Front Immunol. 2012; 3:283. (Biology). View Reference
  2. Dang Q, Liu J, Li J, Sun Y. Podoplanin: a novel regulator of tumor invasion and metastasis.. Med Oncol. 2014; 31(9):24. (Biology). View Reference
  3. Kato Y, Ogasawara S, Oki H, et al. Novel Monoclonal Antibody LpMab-17 Developed by CasMab Technology Distinguishes Human Podoplanin from Monkey Podoplanin.. Monoclon Antib Immunodiagn Immunother. 2016; 35(2):109-16. (Immunogen: Flow cytometry, Immunohistochemistry, Western blot). View Reference
  4. Pan Y, Xia L. Emerging roles of podoplanin in vascular development and homeostasis.. Front Med. 2015; 9(4):421-30. (Biology). View Reference
  5. Ugorski M, Dziegiel P, Suchanski J. Podoplanin - a small glycoprotein with many faces.. Am J Cancer Res. 2016; 6(2):370-86. (Biology). View Reference
940270 Rev. 2

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.