FITC Mouse Anti-Human CD158a

BD™ FITC Mouse Anti-Human CD158a

Name: FITC Mouse Anti-Human CD158a
Brand: BD™
SKU: 340531

Clone HP-3E4

(RUO (GMP))

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Product Details

Brand: BD™

Alternative Name: KIR2DL1; KI2L1; NKAT; NKAT-1; NKAT1; KIR-K64; KIR221; p58.1

Reactivity: Human

Isotype: Mouse BALB/c IgM

Immunogen: Human NK Cells

Application: Flow cytometry

Vol. Per Test: 20 μL

Workshop Number: VI BP373, NKNK14

Storage Buffer: Phosphate buffered saline with gelatin and 0.1% sodium azide.

Regulatory Status: RUO (GMP)

Preparation And Storage

The FITC conjugate is supplied in 1.0 mL of PBS. Buffered saline contains gelatin and 0.1% sodium azide. Please refer to the vial label for antibody concentration. The vial should be stored at 2° to 8°C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.

340531 Rev. 1

Antibody Details

HP-3E4

The CD158a antibody, clone HP-3E4, is derived from the fusion of SP2 mouse myeloma cells with spleen cells isolated from BALB/c mice immunized with activated NK lymphocytes.

The CD158a antibody recognizes a 58-kilodalton (kDa) surface protein found on NK cells and on some CD4– T lymphocytes.

340531 Rev. 1

Format Details

FITC

Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: FITC

Excitation Source: Blue 488 nm

Excitation Max: 494 nm

Emission Max: 518 nm

340531 Rev.1

Citations & References

Development References (4)

Colonna M, Samaridis J. Cloning of immunoglobulin-superfamily members associated with HLA-C and HLA-B recognition by human natural killer cells. Science. 1995; 268(5209):405-408. (Biology). View Reference
Gudelj L, Deniz G, Rukavina D, Johnson PM, Christmas SE. Expression of functional molecules by human CD3- decidual granular leucocyte clones. Immunology. 1996; 87:609-615. (Biology).
Lanier LL, Gumperz JE, Parham P, Melero I, López-Botet M, Phillips JH. The NKB1 and HP-3E4 NK cells receptors are structurally distinct glycoproteins and independently recognize polymorphic HLA-B and HLA-C molecules. J Immunol. 1995; 154(7):3320-3327. (Biology). View Reference
Melero I, Salmeron A, Balboa MA, Aramburu J, Lopez-Botet M. Tyrosine kinase-dependent activation of human NK cell functions upon stimulation through a 58-kDa surface antigen selectively expressed on discrete subsets of NK cells and T lymphocytes. J Immunol. 1994; 152(4):1662-1673. (Biology). View Reference

View All (4)

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Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Although not required, these products are manufactured in accordance with Good Manufacturing Practices.