APC Mouse Anti-Human CD107a
Clone H4A3 (RUO (GMP))
- Brand BD FastImmune™
- Alternative Name LAMP-1
- Vol. Per Test 5 μL
- Isotype Mouse BALB/c IgG1, κ
- Reactivity Human (QC Testing)
Flow cytometry (Routinely Tested)
- Immunogen Human Adult Adherent Peripheral Blood Cells
- Workshop No. V P008
- Regulatory Status RUO (GMP)
Regulatory Status Legend
The BD FastImmune™ CD107a APC reagent is designed for the detection of degranulating T lymphocytes in activated whole blood and is intended for research use only. Applications include studies of cytotoxic CD8+ T-cell responses to viral and tumor antigens, correlation of T-cell cytolytic potential and cytokine expression, and live-cell sorting of functional antigen-specific CD8+ T cells. The CD107a detection assay can also be used as an alternative to 51Cr release assays.
Each vial supplies sufficient reagents for 50 stimulated samples and 50 unstimulated control samples. In performing the assay, 0.5 mL of whole blood is stimulated with antigen (not included) in the presence of two secretion inhibitors, monensin and brefeldin A (BFA), and CD107a antibody. For the unstimulated control, 0.5 mL of blood is treated with the secretion inhibitors and CD107a, but not antigen. Both blood samples are then stained for IFN-γ or other cytokine(s), as well as the gating markers CD3 and CD8 (not included).
Allophycocyanin (APC), is an accessory photosynthetic pigment found in blue-green algae. Its molecular weight is approximately 105 kDa. APC has six phycocyanobilin chromophores per molecule, which makes it a very bright fluorochrome that is highly suitable for flow cytometry applications. Due to nearly identical excitation and emission properties but different spillover characteristics, APC and Alexa Fluor® 647 cannot be used simultaneously
Preparation and Storage
Store vials at 2°C–8°C. Conjugated forms should not be frozen. Protect from exposure to light. Each reagent is stable until the expiration date shown on the bottle label when stored as directed. Alteration in the appearance of any reagent, such as precipitation or discoloration, indicates instability or deterioration. In such cases, do not use the reagent.