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Anti-Human CD3 FITC/Myeloperoxidase (MPO) PE/CD79a PerCP-Cy™5.5

BD Oncomark™ Anti-Human CD3 FITC/Myeloperoxidase (MPO) PE/CD79a PerCP-Cy™5.5

(RUO (GMP))
Product Details
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BD Oncomark™
Human
Flow cytometry
RUO (GMP)
Phosphate buffered saline with BSA and 0.1% sodium azide.


Description

CD3, clone UCHT1, is derived from hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immunized with human thymocytes.

Anti-MPO, clone 5B8, is generated from the fusion of X63.Ag8-653 myeloma cells with spleen cells from BALB/c mice immunized with commercially purified myeloperoxidase.

CD79a, clone HM47, is generated from the fusion of Sp2/0 myeloma cells with spleen cells from BALB/c mice immunized with a synthetic peptide conjugated to thyroglobulin. The peptide had the sequence representing amino acids 202 to 216 inclusive as deduced from the human mb-1 cDNA sequence.

CD3 reacts with the epsilon chain of the CD3 antigen/T-cell antigen receptor (TCR) complex. The antigen recognized by CD3 antibodies is noncovalently associated with either α/β or γ/δ TCR, 70 to 90 kilodaltons (kd).

Anti-MPO reacts with intracellular MPO, a hemoprotein consisting of two molecular subunits, 60 kd and 12 kd. MPO is a major enzyme involved in the inflammatory responses of polymorphonuclear leucocytes. It catalyzes the production of hypochlorous acid, a potent microbicidal agent.

CD79a recognizes the cytoplasmic domain of a 47-kd membrane glycoprotein present on B lymphocytes.

Preparation And Storage

Store vials at 2° to 8°C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.

340961 Rev. 1
Components
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Description Clone Isotype EntrezGene ID
CD3 FITC SK7 IgG1, κ 916
CD79A PerCP-Cy5.5 HM47 IgG1, κ 973
MPO PE 5B8 IgG1, κ 4353
340961 Rev. 1
Citations & References
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Development References (14)

  1. Audrain MA, Baranger TA, Moguilevski N, et al. Anti-native and recombinant myeloperoxidase monoclonals and human autoantibodies. Clin Exp Immunol. 1997; 107(1):127-134. (Biology). View Reference
  2. Clevers H, Alarcón B, Wileman T, Terhorst C. The T cell receptor/CD3 complex: a dynamic protein ensemble. Annual Rev Immunol. 1988; 6:629. (Biology).
  3. Dworzak MN, Fritsch G, Fröschl G, Printz D, Gadner H. Four-color flow cytometric investigation of terminal deoxynucleotidyl transferase-positive lymphoid precursors in pediatric bone marrow: CD79a expression precedes CD19 in early B-cell ontogeny.. Blood. 1998; 92(9):3203-9. (Biology). View Reference
  4. Engel P, Wagner N, Tedder TF. Schlossman SF, Boumsell L, Gilks W, et al, ed. Leucocyte Typing V: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1995:667-679.
  5. Grégoire C, Welch H, Astarie-Dequeker C, Maridonneau-Parini I. Expression of azurophil and specific granule proteins during differentiation of NB4 cells in neutrophils. J Cell Physiol. 1998; 175:203-210. (Biology).
  6. Knapp W, Strobl H, Majdic O. Flow cytometric analysis of cell-surface and intracellular antigens in leukemia diagnosis. Cytometry. 1994; 18(4):187-198. (Biology). View Reference
  7. Mason DY, Cordell JL, Tse AG, et al. The IgM-associated protein mb-1 as a marker of normal and neoplastic B cells. J Immunol. 1991; 147(11):2474-2482. (Biology). View Reference
  8. Nakase K, Sartor M, Bradstock K. Detection of myeloperoxidase by flow cytometry in acute leukemia. Cytometry. 1998; 34:198-202. (Biology).
  9. Nauseef WM, Malech HL. Analysis of the peptide subunits of human neutrophil myeloperoxidase. Blood. 1986; 5:1504-1507. (Biology).
  10. Reichert T, DeBruyere M, Deneys V, et al. Lymphocyte subset reference ranges in adult Caucasians. Clin Immunol Immunopathol. 1991; 60(2):190-208. (Biology). View Reference
  11. Sakaguchi N, Kashiwamura S, Kimoto M, Thalmann P, Melchers F. B lymphocyte lineage-restricted expression of mb-1, a gene with CD3-like structural properties. EMBO J. 1988; 7(11):3457-3464. (Biology). View Reference
  12. Scheinecker C, Strobl H, Fritsch G, et al. Granulomonocyte-associated lysosomal protein expression during in vitro expansion and differentiation of CD34+ hematopoietic progenitor cells. Blood. 1995; 86:4115-4123. (Biology).
  13. Tobler A, Miller CW, Johnson KR, Selsted ME, Rovera G, Koeffler HP. Regulation of gene expression of myeloperoxidase during myeloid differentiation. J Cellular Physiol. 1988; 136:215-225. (Biology).
  14. van Dongen JJM, Krissansen GW, Wolvers-Tettero ILM, et al. Cytoplasmic expression of the CD3 antigen as a diagnostic marker for immature T-cell malignancies. Blood. 1988; 71:603-612. (Biology).
View All (14) View Less
340961 Rev. 1

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