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      Anti-Human CD103 FITC/CD22 PE/CD20 PerCP-Cy™5.5

      BD Oncomark™ Anti-Human CD103 FITC/CD22 PE/CD20 PerCP-Cy™5.5

      (RUO (GMP))
      Product Details
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      BD Oncomark™
      Human
      Flow cytometry
      RUO (GMP)
      Phosphate buffered saline with BSA and 0.1% sodium azide.


      Description

      CD103, clone Ber-ACT 8, is derived from hybridization of spleen cells from BALB/c mice immunized with HTLV-1 positive cell line (MAPS16).

      CD22, clone S-HCL-1, is derived from hybridization of mouse NS-1 myeloma cells with spleen cells from CD-1 mice immunized with whole cells and membrane preparations of hairy-cell leukemia cells.

      CD20, clone L27, is derived from hybridization of mouse Sp2/0 myeloma cells with spleen cells from BALB/c mice immunized with the LB lymphoblastoid cell line.

      CD103 recognizes the αE subunit of integrin αEβ7, an integrin also known as the human mucosa lymphocyte (HML) antigen but not α4, another member of the β7 integrin subfamily. Integrin   αEβ7 is a trimeric protein complex of three components, 105 kilodaltons (kd) (β7), 150 kd, and 25 kd. The CD103 epitope is localized on the 150-kd chain.

      CD22 recognizes a human B-lymphocyte antigen, Mr 135 kd.

      The CD20 antigen is a phosphoprotein with a molecular weight of 35 or 37 kd depending on the degree of phosphorylation. The antigen is not glycosylated.

      Preparation And Storage

      Store vials at 2–8°C. Do not freeze reagents; protect them from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.

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      341122 Rev. 1
      Components
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      Description Clone Isotype EntrezGene ID
      CD22 PE S-HCL-1 IgG2b, κ 933
      CD20 PerCP-Cy™5.5 14D4 IgG2a, κ 12267
      CD103 FITC Ber-ACT8 IgG1, κ 3682
      341122 Rev. 1
      Citations & References
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      Development References (19)

      1. Borowitz MJ, Bousvaros A, Brynes RK, et al. Monoclonal antibody phenotyping of B-cell non-Hodgkin's lymphomas. The Southeastern Cancer Study Group experience.. Am J Pathol. 1985; 121(3):514-21. (Biology). View Reference
      2. Cepek K, Wong D, Brenner M, Springer T. Schlossman S, Boumsell L, Gilks W, et al Leucocyte Typing V: White Cell Differentiation Antigens, ed. New York, NY: 1995:1666-1667.
      3. Dörken B, Möller P, Pezzutto A, Schwartz-Albiez R, Moldenhauer G. KnappW, Dörken B, Gilks WR, et al, ed. Leucocyte Typing IV: White Cell Differentiation Antigens. New York,NY: Oxford University Press; 1989:46-48.
      4. Hoffkes HG, Schmidtke G, Uppenkamp M, Schmucker U. Multiparametric immunophenotyping of B cells in peripheral blood of healthy adults by flow cytometry. Clin Diagn Lab Immunol. 1996; 3:30-36. (Biology).
      5. Hultin LE, Hausner MA, Hultin PM, Giorgi JV. CD20 (pan-B cell) antigen is expressed at a low level on asubpopulation of human T lymphocytes. Cytometry. 1993; 14:196-204. (Biology).
      6. Hübl W, Iturraspe J, Braylan RC. FMC7 antigen expression on normal and malignant B-cells can be predicted by expression of CD20. Cytometry. 1998; 34:71-74. (Biology).
      7. Kruschwitz M, Fritzsche G, Schwarting R, et al. Ber-ACT8: new monoclonal antibody to the mucosa lymphocyte antigen. J Clin Pathol. 1991; 44(8):636-645. (Biology). View Reference
      8. Loken MR, Shah VO, Dattilio K, Civin C. Flow cytometric analysis of human bone marrow, I: Normal erythroid development. Blood. 1987; 69:255-263. (Biology).
      9. Loken MR, Shah VO, Hollander Z, Civin CI. Flow cytometric analysis of normal B lymphoid development.. Pathol Immunopathol Res. 1988; 7(5):357-70. (Biology). View Reference
      10. Mason DY, Stein H, Gerdes J, et al. Value of monoclonal anti-CD22 (p135) antibodies for the detection of normal and neoplastic B lymphoid cells.. Blood. 1987; 69(3):836-40. (Biology). View Reference
      11. Micklem KJ, Dong Y, Willis A, et al. HML-1 antigen on mucosa-associated T cells, activated cells, and hairy leukemic cells is a new integrin containing the β7 subunit. Am J Pathol. 1996; 3:30-36. (Biology).
      12. Nadler LM. B Cell/Leukemia Panel Workshop: summary and comments. In: Reinherz EL, Haynes BF, Nadler LM, Bernstein ID, ed. Leukocyte Typing II: Human B Lymphocytes. New York: Springer-Verlag; 1986:3-43.
      13. Parker CM, Cepek KL, Russell GJ, et al. A family of beta 7 integrins on human mucosal lymphocytes. Proc Natl Acad Sci U S A. 1992; 89(5):1924-1928. (Biology). View Reference
      14. Reeves JE, Robbins BA, Pankey LR, Elias AL, Anderson WF. The simultaneous occurrence of variant hairy-cell leukemia and chronic-phase chronic myelogenous leukemia. A case report. Cancer. 1995; 75(8):2089-2092. (Biology).
      15. Robbins BA, Ellison DJ, Spinosa JC, et al. Diagnostic application of two-color flow cytometry in 161 cases of hairy-cell leukemia. Blood. 1993; 82(4):1277-1287. (Biology).
      16. Schwarting R, Stein H, Wang CY. The monoclonal antibodies alpha S-HCL 1 (alpha Leu-14) and alpha S-HCL 3 (alpha Leu-M5) allow the diagnosis of hairy cell leukemia. Blood. 1985; 65(4):974-983. (Biology). View Reference
      17. Tanaka T, Goda K, Takeuchi E, Miyasaka M. Kishimoto T, Kikutani H, von dem Borne AEGK, ed. Leucocyte Typing VI. White Cell Differentiation Antigens. New York, NY: Garland Publishing, Inc; 1997:430-432.
      18. Terstappen LWMM, Johnsen S, Segers-Nolten IMJ, Loken MR. Identification and characterization of plasma cells in normal human bone marrow by high resolution flow cytometry. Blood. 1990; 76:1739-1747. (Biology).
      19. Tworek JA, Singleton TP, Schnitzer B, Hsi ED, Ross CW. Flow cytometric and immunohistochemical analysis of small lymphocytic lymphoma, mantle cell lymphoma, and plasmacytoid small lymphocytic lymphoma. Am J Clin Pathol. 1998; 110:582-589. (Biology).
      View All (19) View Less
      341122 Rev. 1

      Please refer to Support Documents for Quality Certificates

      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures. 

       

      Although not required, these products are manufactured in accordance with Good Manufacturing Practices.

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