FITC Goat Anti-Rat Ig
Clone Polyclonal (RUO)
- Brand BD Pharmingen™
- Concentration 0.5 mg/ml
- Isotype Goat Ig
- Reactivity Rat (QC Testing)
Flow cytometry (Routinely Tested)
- Storage Buffer Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
- Regulatory Status RUO
Regulatory Status Legend
FITC, fluorescein isothiocyanate, is a fluorochrome with a molecular weight of 389 Da. FITC is sensitive to pH changes and photobleaching. Due to nearly identical excitation and emission properties but different spillover characteristics, FITC and Alexa Fluor® 488 cannot be used simultaneously. FITC is relatively dim and should be reserved for highly expressed markers whenever possible.
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Preparation and Storage
The polyclonal antibody was purified from antiserum by negative adsorption and affinity chromatography.
The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
This antibody conjugate has been tested by immunofluorescent staining (≤ 1 µg/million cells) with flow cytometric analysis on rat splenocytes and human lysed whole blood and as a second-step reagent on mouse splenocytes to assure specificity and reactivity. This antibody stains rat peripheral B cells, and it has little reactivity with rat non-B splenocytes, mouse splenocytes, or human peripheral blood leukocytes. As a second step, it is reactive with rat IgG and IgM monoclonal antibodies; a weaker signal is detected when the primary antibody has a rat IgG2b isotype. It has weak cross-reactivity detectable by flow cytometry with some, but not all, hamster immunoglobulins. Consequently, it may be useful as a primary reagent in immunofluorescent staining of rat antibody-producing cells or as a secondary reagent for staining of mouse or human leukocytes after reaction with rat Ig primary antibodies. However, we have observed that the reactivity of polyclonal second-step antibodies to mouse or rat IgM may be reduced after adsorption against Ig of rat or mouse, respectively. Because this anti-rat Ig antibody was adsorbed with mouse Ig, it may be weakly reactive with some rat IgM primary antibodies. In those cases, we recommend FITC-conjugated anti-rat IgM mAb G53-238 (Cat. no. 553887) or FITC-conjugated anti-rat Ig κ light chain mAb MRK-1 (Cat. no. 553872).