PE Mouse Anti-Dog CD34
Clone 1H6 (RUO)
- Brand BD Pharmingen™
- Concentration 0.2 mg/ml
- Isotype Mouse BALB/c IgG1, κ
- Reactivity Dog (QC Testing)
Flow cytometry (Routinely Tested)
- Immunogen Dog CD34-mouse IgG2a fusion protein and dog myelomonocytic leukemia ML3
- Storage Buffer Aqueous buffered solution containing ≤0.09% sodium azide.
- Regulatory Status RUO
Regulatory Status Legend
The 1H6 antibody reacts with CD34, an ~110 kDa glycoprotein on the surface of bone marrow-derived progenitors of hematopoietic and endothelial cells. In the bone marrow, 1-3% of cells are stained with 1H6 mAb; whereas peripheral blood leukocytes are not stained. Immunomagnetic depletion of lineage-committed leukocytes from bone marrow results in about three-fold enrichment of CD34+ cells. CD34+ hematopoietic progenitors may be mobilized to the peripheral blood by treatment with recombinant canine granulocyte colony-stimulating factor and stem-cell factor. Furthermore, CD34 is expressed on some canine leukemias. In the mouse, CD34 is also expressed on high endothelial venules (HEV) of lymph nodes and, in this form, functions as a ligand for L-selectin. CD34 expression on HEV of dog lymph nodes has been demonstrated with polyclonal anti-CD34 antibody.
- Format PE
- Excitation Source Blue 488 nm,Green 532 nm,Yellow/Green 561 nm
- Excitation Max 496 nm
- Emission Max 578 nm
R-phycoerythrin (PE) is an accessory photosynthetic pigment found in red algae. It exists in vitro as a 240-kDa protein with 23 phycoerythrobilin chromophores per molecule. This makes PE one of the brightest fluorochromes for flow cytometry applications, but its photobleaching properties make it unsuitable for fluorescence microscopy.
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Preparation and Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.