Rat Compensation Set
- Brand BD Pharmingen™
Flow cytometry (Routinely Tested)
- Regulatory Status RUO
Regulatory Status Legend
The Rat Compensation Set is intended to be used with the Rat T lymphocyte Cocktail (Cat. no. 558493) or the Rat T/B/NK Cell Cocktail (Cat. no. 558495) for instrument compensation. Compensation is the process by which the effect of spectral overlap among flurochromes can be corrected minimizing fluorescence signal spillover in neighboring channels. Traditionally, compensation values are entered into software programs allowing for the adjustment among the overlapping flurochrome emissions. These values can be achieved using single color controls, and verified using fluorescence-minus-one controls, or ad-mixtures of single color controls. Use this set to properly adjust your FITC/PE/APC compensation matrix for the Rat T Lymphocyte or Rat T/B/NK Cell Cocktails.
Suggested Companion Products
Preparation and Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
The antibody was conjugated to APC under optimum conditions, and unconjugated antibody and free APC were removed.
The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.