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APC Mouse Anti-Rat CD59
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APC Mouse Anti-Rat CD59
Flow cytometric analysis of CD59 expression on rat bone marrow cells.  Bone marrow cells from a Lewis rat were stained with a BD Horizon™ V450 Mouse Anti-Rat CD45 antibody (Cat. No. 561587) and with either an APC Mouse IgG1, κ Isotype Control (Cat. No. 555751, dashed line histogram) or an APC Mouse Anti-Rat CD59 antibody (Cat. No. 562103, solid line histogram). Flow cytometric fluorescence histograms were derived from events gated on CD45-negative cells. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.
Flow cytometric analysis of CD59 expression on rat bone marrow cells.  Bone marrow cells from a Lewis rat were stained with a BD Horizon™ V450 Mouse Anti-Rat CD45 antibody (Cat. No. 561587) and with either an APC Mouse IgG1, κ Isotype Control (Cat. No. 555751, dashed line histogram) or an APC Mouse Anti-Rat CD59 antibody (Cat. No. 562103, solid line histogram). Flow cytometric fluorescence histograms were derived from events gated on CD45-negative cells. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.
Product Details
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BD Pharmingen™
MACIF; Membrane attack complex inhibition factor; Protectin
Rat (QC Testing)
Mouse BALB/c IgG1, κ
Membrane attack complex-inhibitory proteins from Rat erythrocyte membranes
Flow cytometry (Routinely Tested)
0.2 mg/ml
AB_10894396
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to APC under optimum conditions, and unconjugated antibody and free APC were removed.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  5. This APC-conjugated reagent can be used in any flow cytometer equipped with a dye, HeNe, or red diode laser.
  6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
562103 Rev. 1
Antibody Details
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TH9

The TH9 antibody monoclonal antibody specifically binds to CD59, a 21 kDa glycosyl-phosphatidyl inositol-anchored cell-surface glycoprotein of the Ly-6 superfamily. CD59 is expressed by many types of non-hematopoietic cells. In the rat hematopoietic system, CD59 has been detected on erythrocytes, monocytes, and some lymphocytes, but not on platelets. Soluble CD59 is found in body fluids and urine. CD59 is a complement regulatory protein that acts late in the complement cascade to prevent formation of the membrane attack complex (MAC). Therefore, CD59 is one of several proteins whose function is to protect host tissue from complement attack. Rat CD59 binds rat and human complement components and inhibits cytolysis mediated by complement from multiple species. CD59 has also been suggested to be a ligand for CD2 and to participate in T-cell costimulation.

562103 Rev. 1
Format Details
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APC
Allophycocyanin (APC), is part of the BD family of phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 651 nm and an emission maximum (Em Max) at 660 nm. APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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APC
Red 627-640 nm
651 nm
660 nm
562103 Rev.1
Citations & References
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Development References (6)

  1. Funabashi K, Okada N, Matsuo S, Yamamoto T, Morgan BP, Okada H. Tissue distribution of complement regulatory membrane proteins in rats. Immunology. 1994; 81(3):444-451. (Biology). View Reference
  2. Hughes TR, Piddlesden SJ, Williams JD, Harrison RA, Morgan BP. Isolation and characterization of a membrane protein from rat erythrocytes which inhibits lysis by the membrane attack complex of rat complement. Biochem J. 1992; 284(1):169-176. (Immunogen). View Reference
  3. Lehto T, Morgan BP, Meri S. Binding of human and rat CD59 to the terminal complement complexes. Immunology. 1997; 90(1):121-128. (Biology). View Reference
  4. Liversidge J, Dawson R, Hoey S, McKay D, Grabowski P, Forrester JV. CD59 and CD48 expressed by rat retinal pigment epithelial cells are major ligands for the CD2-mediated alternative pathway of T cell activation. J Immunol. 1996; 156(10):3696-3703. (Clone-specific: (Co)-stimulation, Stimulation). View Reference
  5. Rushmere NK, Tomlinson S, Morgan BP. Expression of rat CD59: functional analysis confirms lack of species selectivity and reveals that glycosylation is not required for function. Immunology. 1997; 90(4):640-646. (Biology). View Reference
  6. Sugita Y, Masuho Y. CD59: its role in complement regulation and potential for therapeutic use. Immunotechnology. 1995; 1(3-4):157-168. (Biology). View Reference
View All (6) View Less
562103 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.