Mouse B Lymphocyte Activation Antibody Cocktail, with Isotype Control; PE-Cy™7 CD25, PE CD69, & APC CD19
- Brand BD Pharmingen™
- Reactivity Mouse (QC Testing)
Flow cytometry (Routinely Tested)
- Regulatory Status RUO
Regulatory Status Legend
The Mouse B Lymphocyte Activation Antibody Cocktail is a three-color reagent designed to identify major subsets of B lymphocytes by direct immunofluorescent staining with flow cytometric analysis. The PC61 antibody reacts with CD25, the low-affinity IL-2 Receptor achain (IL-2Rα, p55) expressed on activated T and B lymphocytes from all mouse strains tested. CD25 is also found on some developing B cells in the bone marrow, early developing T cells in the thymus, peripheral CD4+ regulatory T (Treg) cells, and dendritic cells. The H1.2F3 antibody reacts with CD69 (Very Early Activation antigen). Its expression is rapidly induced upon activation of lymphocytes (T, B, NK, and NK-T cells) neutrophils, and macrophages. CD69 is also expressed on thymocytes that are undergoing positive selection. The 1D3 antibody reacts with CD19, a B lymphocyte-lineage differentiation antigen that is expressed throughout B-lymphocyte development from the pro-B cell through the mature B-cell stages. Terminally differentiated plasma cells do not express CD19. The three antibodies have been titrated and pre-diluted, mixed together, and formulated for optimal staining performance. The Mouse B Lymphocyte Activation Isotype Control contains equivalent concentrations of fluorochrome- and isotype-matched negative-control immunoglobulin.
The use of three different fluorochromes for the labeling of the three different antibodies permits the recognition of each of the three antigens on each cell in a sample. The expression of the three antigens identifies activated B lymphocytes. Additional fluorochrome-labeled reagents may be combined with the Mouse B Lymphocyte Activation Antibody Cocktail, and the Mouse B Lymphocyte Activation Isotype Control, to further characterize activated B-cell subpopulations.
Mouse B Lymphocyte Activation Antibody Cocktail; PE-Cy™7 CD25, PE CD69, and APC CD19
||100 Tests (1 ea)|
Mouse B Lymphocyte Activation Isotype Control; PE-Cy™7, PE, and APC
||100 Tests (1 ea)|
Preparation and Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
The antibody was conjugated with PE-Cy7 under optimum conditions, and unconjugated antibody and free PE-Cy7 were removed.
The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
The antibody was conjugated to APC under optimum conditions, and unconjugated antibody and free APC were removed.
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.