FITC Rat Anti-Mouse Vα 11.1, 11.2[b,d] TCR

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BD Pharmingen™ FITC Rat Anti-Mouse Vα 11.1, 11.2[b,d] TCR

Name: FITC Rat Anti-Mouse Vα 11.1, 11.2[b,d] TCR
Brand: BD Pharmingen™
SKU: 553222

Clone RR8-1

(RUO)

Two-color analysis of the expression of Vα 11.1, 11.2[b,d] TCR on peripheral lymphocytes. C57BL/6 lymph node cells were incubated simultaneously with FITC-conjugated RR8-1, PE-conjugated RM4-5 (anti-CD4, Cat. No. 553048/553049), and PE-conjugated 53-6.7 (anti-CD8a, Cat. No. 553032/553033) monoclonal antibodies. Flow cytometry was performed on a FACScan™ (BDIS, San Jose, CA).

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Product Details

Brand: BD Pharmingen™

Reactivity: Mouse (QC Testing)

Isotype: Rat IgG2b, κ

Immunogen: Mouse T-Cell Clone B10

Application: Flow cytometry (Routinely Tested)

Concentration: 0.5 mg/ml

RRID: AB_394717

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Recommended Assay Procedures

For flow cytometry of cell suspensions from peripheral lymphoid tissues, it is recommended that multicolor staining be performed to distinguish T lymphocytes from non-T cells.

Product Notices

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Companion Products

FITC Rat IgG2b, κ Isotype Control RUO

Size 0.25 mg Cat No. 553988

PE Rat Anti-Mouse CD4 RUO

Size 0.1 mg Cat No. 553048

PE Rat Anti-Mouse CD8a RUO

Size 0.1 mg Cat No. 553032

553222 Rev. 9

Antibody Details

RR8-1

The RR8-1 monoclonal antibody specifically recognizes the Vα 11.1 and Vα 11.2, but not Vα 11.3 T-cell Receptors (TCR) of mice having the b (e.g., C57BL) and d (e.g., DBA/1, DBA/2, NZW) haplotypes of Tcra gene complex. RR8-1 antibody does not react with strains having the a (e.g., A, AKR, BALB/c, CBA, C3H/He) or c (e.g., NZB, SJL, SWR, NOD) Tcra haplotypes. Plate-bound RR8-1 antibody activates Vα 11.1, 11.2[b,d] TCR-bearing T lymphocytes.

This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

553222 Rev. 9

Format Details

FITC

Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: FITC

Excitation Source: Blue 488 nm

Excitation Max: 494 nm

Emission Max: 518 nm

553222 Rev.9

Citations & References

Development References (1)

Jameson SC, Nakajima PB, Brooks JL, Heath W, Kanagawa O, Gascoigne NR. The T cell receptor V alpha 11 gene family. Analysis of allelic sequence polymorphism and demonstration of J alpha region-dependent recognition by allele-specific antibodies. J Immunol. 1991; 147(9):3185-3193. (Immunogen). View Reference

553222 Rev. 9

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.