FITC Mouse Anti-Mouse Vβ 17[a] T-Cell Receptor
Clone KJ23 (RUO)
- Brand BD Pharmingen™
- Concentration 0.5 mg/ml
- Isotype Mouse BALB/c IgG2a, κ
- Reactivity Mouse (QC Testing)
- Application
Flow cytometry (Routinely Tested)
- Immunogen Mouse T cells
- Storage Buffer Aqueous buffered solution containing ≤0.09% sodium azide.
- Regulatory Status RUO
Regulatory Status Legend
Description
The KJ23 monoclonal antibody specifically recognizes Vβ 17[a] T-cell Receptor (TCR) of mice having the a haplotype (eg,C57L, SJL, SWR) of the Tcrb gene complex. Strains having the b (eg, A, AKR, BALB/c, CBA,C3H/He, C57BL, C58, DBA/1, DBA/2) Tcrb haplotype do not express functional Vβ 17 TCR, and the Tcrb-V17 gene locus is deleted in mice having the c (eg, RIII) haplotype. Vβ 17[a] TCR-bearing Tlymphocytes are clonally eliminated in mice expressing I-E (eg, C57BR). KJ23 antibody also recognizes two phenotypic variants of the Vβ 17[a] TCR: Vβ 17[a2] expressed in a variety of wild-derivedmouse strains and Vβ 17[a(cz)] expressed in Mtv-free CZ mice. The effects of Mtv-encoded superantigens upon Vβ 17[a] TCR-bearing T cells has been reviewed. Plate-bound KJ23 antibody activates Vβ 17[a] TCR-bearing T cells, and injection of the antibody can deplete Vβ 17[a]-bearing Tcells.
Format
FITC, fluorescein isothiocyanate, is a fluorochrome with a molecular weight of 389 Da. FITC is sensitive to pH changes and photobleaching. Due to nearly identical excitation and emission properties but different spillover characteristics, FITC and Alexa Fluor® 488 cannot be used simultaneously. FITC is relatively dim and should be reserved for highly expressed markers whenever possible.
Suggested Companion Products
PE Rat Anti-Mouse CD8a 53-6.7 RUO
0.1 mg
Cat No: 553032
PE Rat Anti-Mouse CD4 RM4-5 (also known as RM4.5) RUO
0.1 mg
Cat No: 553048
FITC Mouse IgG2a, κ Isotype Control RUO
0.25 mg
Cat No: 553456
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Preparation and Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
For flow cytometry of cell suspensionsfrom peripheral lymphoid tissues, it is recommended that multicolor staining be performed todistinguish T lymphocytes from non-T cells.