BV650 Mouse Anti-Human PI-16
Clone CRCBT-02-001 (RUO)
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- Alternative Name CD364; PI16; Peptidase inhibitor 16; CRISP-9; PSPBP; PSP94-binding protein
- Concentration 0.2 mg/ml
- Isotype Mouse IgG1, κ
- Reactivity Human (Tested in Development)
Flow cytometry (Qualified)
- Immunogen Human PI-16 Transfected Cell Line
- Storage Buffer Aqueous buffered solution containing ≤0.09% sodium azide.
- Regulatory Status RUO
Regulatory Status Legend
The CRCBT-02-001 monoclonal antibody specifically binds to human CD364 which is also known as Peptidase inhibitor 16 (PI-16) or Protease inhibitor 16. PI-16 is a glycophosphatidylinositol-linked, 436 amino acid surface glycoprotein. It is a member of the cysteine-rich secretory protein (CRISP) family and is likewise known as CRISP9. PI-16 is a putative serine protease inhibitor and can serve as a binding protein for PSP94 (prostate secretory protein of 94 amino acids) termed PSPBP. Serum levels of PSP94 and PI-16 can reportedly serve as key targets for prostate cancer research. PI-16 is highly expressed by the activated/memory, FoxP3-bright subsets of regulatory T cells. PI-16-positive regulatory T cells expressed uniformly high levels of the chemokine receptors, CCR4 and CCR6, when compared with PI-16-negative T regulatory cells. The results suggest that the CCR4+CCR6+ regulatory T cells are capable of homing to inflammatory sites and regulating effector T cells such as CCR4+CCR6+ Th17-like cells. PI-16 is also expressed by subsets of memory CD4+ and CD8-bright T cells. It is not expressed by B cells or monocytes.
The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 650-nm. BD Horizon BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20-nm filter). Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor® 700 detectors. However, the spillover can be corrected through compensation as with any other dye combination.
BV650 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an emission maximum at 650 nm. Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor® 700 detectors. BV650 will have moderate spillover into the BD Horizon™ BV711 detector.
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Preparation and Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
The antibody was conjugated with BD Horizon BV650 under optimal conditions that minimize unconjugated dye and antibody.
- This antibody was developed for use in flow cytometry.
- The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
- Researchers should determine the optimal concentration of this reagent for their individual applications.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
- BD Horizon Brilliant Violet 650 is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,227,187; 8,455,613; 8,575,303; 8,354,239.
- Alexa Fluor® is a registered trademark of Life Technologies Corporation.
For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes (including BD OptiBuild Brilliant reagents) are used in the same experiment. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794).