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Biotin Rat IgG2a κ Isotype Control RUO

Biotin Rat IgG2a κ Isotype Control 

Clone  R35-95   (RUO)

  • Brand BD Pharmingen™
  • Concentration 0.5 mg/ml
  • Isotype Rat LOU, also known as Louvain, LOU/C, LOU/M IgG2a, κ
  • Application Flow cytometry, Isotype control (Routinely Tested) 
    Immunohistochemistry (Tested During Development) 
  • Immunogen Mouse Pooled Immunoglobulin
  • Storage Buffer Aqueous buffered solution containing ≤0.09% sodium azide.
  • Regulatory Status RUO
Product Details Recommended Assay References

Description

The R35-95 hybridoma was generated by hybridization of Y3 myeloma cells with spleen cells from LOU rats immunized with mouse immunoglobulins. The R35-95 hybridoma produces rat IgG2a, κ immunoglobulin that has no measurable reactivity with mouse immunoglobulins. The R35-95 immunoglobulin was selected as an isotype control following screening for low background binding on a variety of mouse and human tissues.

This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only.

Format
  • Format Biotin

Other Formats →

Suggested Companion Products

Purified Rat IgG2a κ Isotype Control RUO
0.25 mg Cat No: 559073

Avidin FITC RUO
0.5 mg Cat No: 554057

Resources & Tools
 Spectrum Viewer  Download TDS  Regulatory Document Website
Preparation and Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.


An isotype control should be used at the same concentration as the antibody of interest (e.g., ≤ 1 µg/million cells for flow cytometry).

This antibody is useful as an isotype-matched negative control for immunofluoresent and immunohistochemical staining (IHC). For IHC, we recommend the use of purified R35-95 mAb in our special formulation for immunohistochemistry, Cat. No. 559703.

553928 Rev. 15
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