APC Rat Anti-Mouse LPAM-1

This SKU will be discontinuing Apr 2024. Suggested alternate SKU is [564394] or for additional support, contact your local applications specialist. Contact Us

BD Pharmingen™ APC Rat Anti-Mouse LPAM-1

Name: APC Rat Anti-Mouse LPAM-1
Brand: BD Pharmingen™
SKU: 562376

Clone DATK32

(RUO)

Multicolor flow cytometric analysis of LPAM-1 expression on C57BL/6 mouse bone marrow cells. Bone marrow cells were stained with FITC Rat Anti-Mouse CD45R/B220 antibody (Cat. No. 553087/553088/561877) and with either APC Rat IgG2a, κ Isotype Control (Cat. No. 553932; Left Panel) or APC Rat Anti-Mouse LPAM-1 (Cat. No. 562376; Right Panel). Two-color flow cytometric dot plots show the correlated expression patterns of LPAM-1 (or Ig isotype control staining) versus CD45R/B220 for gated events with the forward and side light-scatter characteristics of viable bone marrow cells. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.

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Product Details

Brand: BD Pharmingen™

Alternative Name: Itga4Itgb7; α4β7 Integrin; alpha 4 beta 7 integrin; CD49dβ7

Reactivity: Mouse (QC Testing)

Isotype: Rat F344, also known as Fischer, CDF IgG2a, κ

Immunogen: AKR/Cum mouse T lymphoma line TK1

Application: Flow cytometry (Routinely Tested)

Concentration: 0.2 mg/ml

RRID: AB_11154232

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to APC under optimum conditions, and unconjugated antibody and free APC were removed.

Product Notices

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
An isotype control should be used at the same concentration as the antibody of interest.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
This APC-conjugated reagent can be used in any flow cytometer equipped with a dye, HeNe, or red diode laser.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.

Companion Products

Stain Buffer (FBS) RUO

Size 500 mL Cat No. 554656

APC Rat IgG2a κ Isotype Control RUO

Size 0.1 mg Cat No. 553932

FITC Rat Anti-Mouse CD45R/B220 RUO

Size 0.1 mg Cat No. 553087

FITC Rat Anti-Mouse CD45R/B220 RUO

Size 0.5 mg Cat No. 553088

FITC Rat Anti-Mouse CD45R/B220 RUO

Size 25 µg Cat No. 561877

562376 Rev. 1

Antibody Details

DATK32

The DATK32 monoclonal antibody specifically binds to an epitope specific for the α4β7 integrin heterodimer. α4β7 (LPAM-1) is expressed on most mature lymphocytes and on small subsets of thymic and bone marrow cells. It interacts with several ligands, including VCAM-1 (CD106), fibronectin, and MAdCAM-1. DATK32 antibody induces α4β7-dependent lymphocyte aggregation, but it inhibits other α4β7- mediated lymphocyte adhesion events, including binding to fibronectin, MAdCAM-1, and VCAM-1 (CD106).

562376 Rev. 1

Format Details

APC

Allophycocyanin (APC), is part of the BD family of phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 651 nm and an emission maximum (Em Max) at 660 nm. APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: APC

Excitation Source: Red 627-640 nm

Excitation Max: 651 nm

Emission Max: 660 nm

562376 Rev.1

Citations & References

Development References (5)

Andrew DP, Berlin C, Honda S, et al. Distinct but overlapping epitopes are involved in alpha 4 beta 7-mediated adhesion to vascular cell adhesion molecule-1, mucosal addressin-1, fibronectin, and lymphocyte aggregation. J Immunol. 1994; 153(9):3847-3861. (Immunogen: Blocking, Inhibition). View Reference
Andrew DP, Rott LS, Kilshaw PJ, Butcher EC. Distribution of alpha 4 beta 7 and alpha E beta 7 integrins on thymocytes, intestinal epithelial lymphocytes and peripheral lymphocytes. Eur J Immunol. 1996; 26(4):897-905. (Clone-specific: Blocking). View Reference
Berlin C, Berg EL, Briskin MJ, et al. Alpha 4 beta 7 integrin mediates lymphocyte binding to the mucosal vascular addressin MAdCAM-1. Cell. 1993; 74(1):185-195. (Clone-specific: Blocking). View Reference
Hamann A, Andrew DP, Jablonski-Westrich D, Holzmann B, Butcher EC. Role of alpha 4-integrins in lymphocyte homing to mucosal tissues in vivo. J Immunol. 1994; 152(7):3282-3293. (Clone-specific). View Reference
Kilshaw PJ, Murant SJ. Expression and regulation of beta 7(beta p) integrins on mouse lymphocytes: relevance to the mucosal immune system. Eur J Immunol. 1991; 21(10):2591-2597. (Biology). View Reference

View All (5)

562376 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.