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      Alexa Fluor® 647 Rat Anti-Mouse CD6
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      This SKU will be discontinuing Apr 2024. For additional support, contact your local applications specialist. Contact Us #
      Alexa Fluor® 647 Rat Anti-Mouse CD6
      Two-color flow cytometric analysis of CD6 expression by mouse splenocytes. C57BL/6 mouse splenic leucocytes were stained with BD Horizon™ BV421 Hamster Anti-Mouse CD3e antibody (Cat. No. 562600) and either Alexa Fluor® 647 Rat IgG2a, λ Isotype Control (Cat. No. 564281; Left Plot) or Alexa Fluor® 647 Rat Anti-Mouse CD6 antibody (Cat. No. 566426; Right Plot) at 0.06 µg/test. A two-color flow cytometric contour plot showing the correlated expression of CD3e versus CD6 (or Ig Isotype control staining) was derived from gated events with the forward and side-light scattering characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD FACSCanto™ II Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific.
      Alexa Fluor® 647 Rat Anti-Mouse CD6
      Two-color flow cytometric analysis of CD6 expression by mouse splenocytes. C57BL/6 mouse splenic leucocytes were stained with BD Horizon™ BV421 Hamster Anti-Mouse CD3e antibody (Cat. No. 562600) and either Alexa Fluor® 647 Rat IgG2a, λ Isotype Control (Cat. No. 564281; Left Plot) or Alexa Fluor® 647 Rat Anti-Mouse CD6 antibody (Cat. No. 566426; Right Plot) at 0.06 µg/test. A two-color flow cytometric contour plot showing the correlated expression of CD3e versus CD6 (or Ig Isotype control staining) was derived from gated events with the forward and side-light scattering characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD FACSCanto™ II Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific.
      Product Details
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      BD Pharmingen™
      Cd6; CD6 antigen
      Mouse (QC Testing)
      Rat IgG2a, λ
      Mouse CD6 Peptide
      Flow cytometry (Routinely Tested)
      0.2 mg/ml
      12511
      AB_2739726
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.
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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. An isotype control should be used at the same concentration as the antibody of interest.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      4. The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
      5. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
      6. Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
      7. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      8. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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      566426 Rev. 1
      Antibody Details
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      J90-462

      The J90-462 monoclonal antibody specifically recognizes CD6. CD6 is a 105-130 kDa type I transmembrane glycoprotein that belongs to the scavenger receptor family. It is expressed on most thymocytes and peripheral T cells. CD6 is highly expressed on CD3-high thymocytes and expressed at lower levels on CD4+ CD8+ double positive and CD4+CD8- or CD4-CD8+ single positive thymocytes. CD6 is also reportedly expressed at low levels by some neurons.  CD6 serves as a receptor for CD166, also known as Activated Leukocyte Cell Adhesion Molecule (ALCAM). CD6 plays a role in adhesion and migration during organogenesis. The CD6 intracellular domain is constitutively phosphorylated which suggests that CD6 may play a signaling role in T cell activation.

              

      566426 Rev. 1
      Format Details
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      Alexa Fluor™ 647
      Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 520-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      Alexa Fluor™ 647
      Red 627-640 nm
      653 nm
      669 nm
      566426 Rev.1
      Citations & References
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      Development References (6)

      1. Bowen MA, Bajorath J, D'Egidio M, et al. Characterization of mouse ALCAM (CD166): the CD6-binding domain is conserved in different homologs and mediates cross-species binding.. Eur J Immunol. 1997; 27(6):1469-78. (Biology). View Reference
      2. Pal A, Romain PL, Singer NG, Fox D, Stavnezer J. Mouse CD6: sequence of cDNA and expression of mRNA.. Immunol Lett. 1996; 49(1-2):133-7. (Biology). View Reference
      3. Roncagalli R, Hauri S, Fiore F, et al. Quantitative proteomics analysis of signalosome dynamics in primary T cells identifies the surface receptor CD6 as a Lat adaptor-independent TCR signaling hub.. Nat Immunol. 2014; 15(4):384-92. (Biology). View Reference
      4. Singer NG, Fox DA, Haqqi TM, et al. CD6: expression during development, apoptosis and selection of human and mouse thymocytes.. Int Immunol. 2002; 14(6):585-97. (Biology). View Reference
      5. Starling GC, Whitney GS, Siadak AW, et al. Characterization of mouse CD6 with novel monoclonal antibodies which enhance the allogeneic mixed leukocyte reaction.. Eur J Immunol. 1996; 26(4):738-46. (Biology). View Reference
      6. Whitney G, Bowen M, Neubauer M, Aruffo A. Cloning and characterization of murine CD6.. Mol Immunol. 1995; 32(1):89-92. (Biology). View Reference
      View All (6) View Less
      566426 Rev. 1

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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