Multicolor flow cytometric analysis of CD270 (HVEM) expression on mouse bone marrow cells and splenocytes. Left Panel. BALB/c mouse bone marrow cells were stained with APC-Cy™7 CD45R/B220 antibody (Cat. No.552094/561102) and either Alexa Fluor® 647 Rat IgG1, κ Isotype Control (Cat. No. 557731; Left Plot) or Alexa Fluor® 647 Rat Anti-Mouse CD270 (HVEM) (Cat. No. 564470; Right Plot). Two-color flow cytometric contour plots showing the correlated expression patters of CD45R/B220 versus either CD270 (HVEM) or Ig Isotype control staining were derived from gated events with the forward and side light-scattering properties of viable bone marrow cells. Right Panel. BALB/c splenic leucocytes were stained with BD Horizon™ V500 Syrian Hamster Anti-Mouse CD3e antibody (Cat. No. 560771/560773) and either Alexa Fluor® 647 Rat IgG1, κ Isotype Control (Left Plot) or Alexa Fluor® 647 Rat Anti-Mouse CD270 (HVEM) (Right Plot). Two-color flow cytometric contour plots showing the correlated expression patters of CD3e versus either CD270 (HVEM) or Ig Isotype control staining were derived from gated events with the light-scattering properties of viable splenic leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.
Multicolor flow cytometric analysis of CD270 (HVEM) expression on mouse bone marrow cells and splenocytes. Left Panel. BALB/c mouse bone marrow cells were stained with APC-Cy™7 CD45R/B220 antibody (Cat. No.552094/561102) and either Alexa Fluor® 647 Rat IgG1, κ Isotype Control (Cat. No. 557731; Left Plot) or Alexa Fluor® 647 Rat Anti-Mouse CD270 (HVEM) (Cat. No. 564470; Right Plot). Two-color flow cytometric contour plots showing the correlated expression patters of CD45R/B220 versus either CD270 (HVEM) or Ig Isotype control staining were derived from gated events with the forward and side light-scattering properties of viable bone marrow cells. Right Panel. BALB/c splenic leucocytes were stained with BD Horizon™ V500 Syrian Hamster Anti-Mouse CD3e antibody (Cat. No. 560771/560773) and either Alexa Fluor® 647 Rat IgG1, κ Isotype Control (Left Plot) or Alexa Fluor® 647 Rat Anti-Mouse CD270 (HVEM) (Right Plot). Two-color flow cytometric contour plots showing the correlated expression patters of CD3e versus either CD270 (HVEM) or Ig Isotype control staining were derived from gated events with the light-scattering properties of viable splenic leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.
Product Details
BD Pharmingen™
HVEM; Herpes virus entry mediator; Tnfrsf14; HveA; Atar
Mouse (QC Testing)
Rat IgG1, κ
Mouse HVEM Transfected Cell Line
Flow cytometry (Routinely Tested)
0.2 mg/ml
AB_2738818
Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
- Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
- Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Cy is a trademark of GE Healthcare.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
Stain Buffer (FBS) RUO
Size
500 mL
Cat No.
554656
Stain Buffer (BSA) RUO
Size
500 mL
Cat No.
554657
Lysing Buffer RUO
Size
100 mL
Cat No.
555899
Alexa Fluor® 647 Rat IgG1, κ Isotype Control RUO
Size
0.1 mg
Cat No.
557731
V500 Syrian Hamster anti-Mouse CD3e RUO
Size
25 µg
Cat No.
560773
APC-Cy™7 Rat Anti-Mouse CD45R RUO
Size
0.1 mg
Cat No.
552094
564470 Rev. 2
Antibody Details
C46
The C46 monoclonal antibody specifically binds to HVEM (Herpes virus entry mediator) which is also known as CD270. CD270 is a type I transmembrane protein and member of the TNF Receptor superfamily. It is encoded by Tnfrsf14 (tumor necrosis factor receptor superfamily member 14). CD270 is expressed by a variety of cell types including T cells, B cells, monocytes, dendritic cells, neutrophils, and mucosal epithelial cells. CD270 is a multifunctional factor that can both augment or inhibit immune responses. CD270 binds several ligands including CD258/LIGHT (is homologous to lymphotoxins, exhibits inducible expression, and competes with HSV glycoprotein D for HVEM, a receptor expressed by T lymphocytes), CD160, and CD272/BTLA. CD270-LIGHT interactions reportedly transduce costimulatory signals for T cells whereas CD270 interaction with BTLA can induce coinhibitory signals that regulate T cell-dependent immune responses.
564470 Rev. 2
Format Details
Alexa Fluor™ 647
Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 520-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Alexa Fluor™ 647
Red 627-640 nm
653 nm
669 nm
564470 Rev.2
Citations & References
Development References (5)
-
Hsu H, Solovyev I, Colombero A, Elliott R, Kelley M, Boyle WJ. ATAR, a novel tumor necrosis factor receptor family member, signals through TRAF2 and TRAF5. J Biol Chem. 1997; 272(2):13471. (Biology). View Reference
-
Sedy JR, Gavrieli M, Potter KG, et al. B and T lymphocyte attenuator regulates T cell activation through interaction with herpesvirus entry mediator. Nat Immunol. 2005; 6(1):90-98. (Biology). View Reference
-
Shui JW, Larange A, Kim G, et al. HVEM signalling at mucosal barriers provides host defence against pathogenic bacteria. Nature. 2012; 488(7410):222-225. (Biology). View Reference
-
Spear PG. Herpes simplex virus: receptors and ligands for cell entry. Cell Microbiol. 2004; 6(5):401-410. (Biology). View Reference
-
Tamada K, Shimozaki K, Chapoval AI, . LIGHT, a TNF-like molecule, costimulates T cell proliferation and is required for dendritic cell-mediated allogeneic T cell response. J Immunol. 2000; 164(8):4105-4110. (Biology). View Reference
564470 Rev. 2
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
