Two-color flow cytometric analysis of mouse CD161b expression on C57BL/6 mouse splenocytes. Mouse splenic leucocytes were stained with BV421 Mouse Anti-Mouse NK1.1 antibody (Cat. No. 562921) and either Alexa Fluor® 647 Rat IgG2a, κ Isotype Control (Cat. No. 557690; Left Plot) or Alexa Fluor® 647 Rat Anti-Mouse CD161a antibody (Cat. No. 566307; Right Plot). Two-color flow cytometric contour plots showing the correlated expression of NK1.1 versus CD161b (or Ig Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of viable splenocytes. Flow cytometric analysis was performed using a BD FACSCanto™ II Flow Cytometer System.
Two-color flow cytometric analysis of mouse CD161b expression on C57BL/6 mouse splenocytes. Mouse splenic leucocytes were stained with BV421 Mouse Anti-Mouse NK1.1 antibody (Cat. No. 562921) and either Alexa Fluor® 647 Rat IgG2a, κ Isotype Control (Cat. No. 557690; Left Plot) or Alexa Fluor® 647 Rat Anti-Mouse CD161a antibody (Cat. No. 566307; Right Plot). Two-color flow cytometric contour plots showing the correlated expression of NK1.1 versus CD161b (or Ig Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of viable splenocytes. Flow cytometric analysis was performed using a BD FACSCanto™ II Flow Cytometer System.
Product Details
BD Pharmingen™
CD161b; CD161d; Nkrp1b; Nkrp1d; Lymphocyte antigen 55d; Ly55d
Mouse (QC Testing)
Rat PVG, also known as HO, Black Hooded IgG2a, λ
Mouse NKRP1D Transfected Cell Line
Flow cytometry (Routinely Tested)
0.2 mg/ml
AB_2739675
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
- Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
- Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
- This product is provided under an intellectual property license between Life Technologies Corporation and BD Businesses. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for Commercial Purposes. Commercial Purposes means any activity by a party for consideration and may include, but is not limited to: (1) use of the product or its components in manufacturing; (2) use of the product or its components to provide a service, information, or data; (3) use of the product or its components for therapeutic, diagnostic or prophylactic purposes; or (4) resale of the product or its components, whether or not such product or its components are resold for use in research. For information on purchasing a license to this product for any other use, contact Life Technologies Corporation, Cell Analysis Business Unit Business Development, 29851 Willow Creek Road, Eugene, OR 97402, USA, Tel: (541) 465-8300. Fax: (541) 335-0504.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
Stain Buffer (BSA) RUO
Size
500 mL
Cat No.
554657
Stain Buffer (FBS) RUO
Size
500 mL
Cat No.
554656
Lysing Buffer RUO
Size
100 mL
Cat No.
555899
Alexa Fluor® 647 Rat IgG2a, κ Isotype Control RUO
Size
0.1 mg
Cat No.
557690
BV421 Mouse Anti-Mouse NK-1.1 RUO
Size
50 µg
Cat No.
562921
566307 Rev. 4
Antibody Details
2D9
The 2D9 monoclonal antibody recognizes C57BL/6 mouse Natural killer cell receptor P1D (NKR-P1D) which is also known as NKR-P1B[B6], or CD161b. CD161b is encoded by Klrb1b (killer cell lectin-like receptor subfamily B member 1B). CD161b is expressed by a functionally distinct subset of natural killer (NK) cells that express higher levels of cytotoxicity and interferon-γ (IFN-γ) than their CD161b-low or -negative counterparts. CD161b is a type II transmembrane glycoprotein that contains an immunoreceptor tyrosine-based inhibitory motif in its cytoplasmic domain. CD161b binds to C-type lectin related protein b (Clr-b) and may play a role in regulating NK cell responses. The 2D9 antibody has slight crossreactivity with cells transfected with Klrb1a/NKRP1A, Klrb1c/NKRP1C, or Klrb1f/NKRP1F.
566307 Rev. 4
Format Details
Alexa Fluor™ 647
Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 520-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Alexa Fluor™ 647
Red 627-640 nm
653 nm
669 nm
566307 Rev.4
Citations & References
Development References (6)
-
Aust JG1, Gays F, Mickiewicz KM, Buchanan E, Brooks CG.. The expression and function of the NKRP1 receptor family in C57BL/6 mice.. J Immunol. 2009; 183(1):106-116. (Immunogen: Blocking, Flow cytometry, Fluorescence activated cell sorting). View Reference
-
Giorda R, Trucco M. Mouse NKR-P1. A family of genes selectively coexpressed in adherent lymphokine-activated killer cells. J Immunol. 1991; 147(5):1701-1708. (Biology). View Reference
-
Kirkham CL, Carlyle JR. Complexity and Diversity of the NKR-P1:Clr (Klrb1:Clec2) Recognition Systems. Front Biosci. 2014; 5(5):1-16. (Biology). View Reference
-
Kung SK, Su RC, Shannon J, Miller RG. The NKR-P1B gene product is an inhibitory receptor on SJL/J NK cells. J Immunol. 1999; 162(10):5876-5887. (Biology). View Reference
-
Plougastel B, Matsumoto K, Dubbelde C, Yokoyama WM. Analysis of a 1-Mb BAC contig overlapping the mouse Nkrp1 cluster of genes: cloning of three new Nkrp1 members, Nkrp1d, Nkrp1e, and Nkrp1f.. Immunogenetics. 2001; 53(7):592-8. (Biology). View Reference
-
Rozbeský D, Ivanova L, Hernychová L, Grobárová V, Novák P, Černý J. Nkrp1 family, from lectins to protein interacting molecules.. Molecules. 2015; 20(2):3463-78. (Biology). View Reference
566307 Rev. 4
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
