Alexa Fluor® 647 Rat Anti-Human TRAF1
Clone 1F3 (also known as TRAF1-1F3) (RUO)
- Brand BD Pharmingen™
- Alternative Name TRAF1; EBI6; Epstein-Bar virus-induced protein 6
- Concentration 0.2 mg/ml
- Isotype Rat LOU, also known as Louvain, LOU/C, LOU/M IgG2a, κ
- Reactivity Human (QC Testing)
Intracellular staining (flow cytometry) (Routinely Tested)
- Immunogen Human TRAF1 Recombinant Protein
- Storage Buffer Aqueous buffered solution containing ≤0.09% sodium azide.
- Regulatory Status RUO
Regulatory Status Legend
The 1F3 monoclonal antibody specifically recognizes TNF Receptor-associated factor 1 (TRAF1) which is also known as Epstein-Bar virus-induced protein 6 (EBI6). TRAF1 is a ~46 kDa cytoplasmic protein that is encoded by the TRAF1 gene and belongs to the TRAF family of proteins that serve as signaling adaptors. TRAF1 contains a C-terminal TRAF domain that allows it to interact with the cytoplasmic domains of different members of the TNF Receptor superfamily including TNFR1/CD120a, TNFR2/CD120b, CD30, CD40, and RANK/CD265. These interactions contribute to the activation of MAP kinases (eg, JNK) and transcription factors (eg, NF-κB) that participate in regulating cellular proliferation, differentiation, stress responses, and survival. TRAF1 is expressed in cells derived from various tissues including testis, epidermis, and thymus, and at lower levels by resting leucocytes from secondary lymphoid tissues. Leucocyte TRAF1 expression can be upregulated or downregulated in response to different stimuli including infectious agents, mitogens, lipopolysaccharide (LPS), cytokines and other inflammatory mediators.
Alexa Fluor® 647 conjugates are highly photostable and remain fluorescent over a broad pH range. The excitation and emission maxima are nearly identical to those of APC. However, APC tends to be brighter while Alexa Fluor® 647 is more optimal for intracellular applications. This fluorochrome exhibits uncommon photostability, making it an ideal choice for use in fluorescence microscopy. Due to nearly identical excitation and emission properties but different spillover characteristics, APC and Alexa Fluor® 647 cannot be used simultaneously.
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Preparation and Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
- Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
- Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.