Alexa Fluor® 647 Mouse Anti-Human DAP12
Clone 406288 (RUO)
- Brand BD Pharmingen™
- Alternative Name DNAX-activation protein 12; KAR-associated protein; KARAP; PLOSL; TYROBP
- Concentration 0.2 mg/ml
- Isotype Mouse IgG1, κ
- Reactivity Human (QC Testing)
Intracellular staining (flow cytometry) (Routinely Tested)
- Immunogen Human
- Storage Buffer Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
- Regulatory Status RUO
Regulatory Status Legend
The 406288 monoclonal antibody specifically recognizes DNAX-activation Protein 12 (DAP12) which is also known as Killer-activating receptor-associated protein (KARAP) or PLOSL. DAP12 is encoded by TYROBP (TYRO protein tyrosine kinase binding protein). The 12 kDa DAP12 protein contains a short extracellular domain, a transmembrane domain, and a cytoplasmic domain that contains a single immunoreceptor tyrosine-based activation motif (ITAM). DAP12 exists as a disulfide-linked homodimeric protein that is expressed by a variety of hematopoietic cells, including NK cells, monocytes, macrophages, dendritic cells, neutrophils, eosinophils, basophils, mast cells, and some NKT cells and γδ T cells. DAP12 functions as a signal transducing adaptor protein that associates with a variety of receptors involved in the detection of pathogens or host damage, including CD158 members of the Killer cell immunoglobulin like receptors (KIR) gene family, CD94-NKG2C and CD94-NKG2E heterodimeric receptors, Triggering receptor expressed on monocytes 1 (TREM-1) and TREM-2, and the NKp44 (CD336) receptor. These associations are mediated by intramembranal ionic attraction between a DAP12 aspartic acid residue and a lysine residue in the cognate receptor. The association between these receptors leads to either cellular activation or inhibition through dual tyrosine phosphorylation of the ITAM sequence by Src family kinases and further downstream signaling.
Alexa Fluor® 647 conjugates are highly photostable and remain fluorescent over a broad pH range. The excitation and emission maxima are nearly identical to those of APC. However, APC tends to be brighter while Alexa Fluor® 647 is more optimal for intracellular applications. This fluorochrome exhibits uncommon photostability, making it an ideal choice for use in fluorescence microscopy. Due to nearly identical excitation and emission properties but different spillover characteristics, APC and Alexa Fluor® 647 cannot be used simultaneously.
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Preparation and Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
- Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
- Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.