Purified Rat Anti-Human CD132
Clone TUGh4 (RUO)
- Brand BD Pharmingen™
- Alternative Name IL-2RG; IL-2Rγ; γc; Common γ chain; Common gamma chain; gamma c; SCIDX1
- Concentration 0.5 mg/ml
- Isotype Rat IgG2b, κ
- Reactivity Human (QC Testing) Dog (Tested in Development)
Flow cytometry (Routinely Tested)
- Immunogen Human γc Transfected Cell Line
- Workshop No. VI C-89
- Entrez Gene ID 3561
- Storage Buffer Aqueous buffered solution containing ≤0.09% sodium azide.
- Regulatory Status RUO
Regulatory Status Legend
The TUGh4 monoclonal antibody specifically binds to CD132. CD132 is a 65-70 kDa type 1 transmembrane glycoprotein that is encoded by the IL2RG (interleukin 2 receptor, gamma) gene. CD132 is also known as the common γ subunit (γc) and is shared by the IL-2, IL-4, IL-7, IL-9, IL-15 and IL-21 receptor complexes. CD132 is broadly expressed by most peripheral T and B lymphocytes, NK cells, monocytes, and granulocytes. The cytoplasmic domain of the γc chain plays an important role in cytokine-mediated signal transduction. Mutation of the IL2RG gene results in X-linked severe combined immunodeficiency (XSCID). The TUGh4 antibody recognizes a different epitope from that recognized by the CD132-specific clone, AG184.
Suggested Companion Products
Preparation and Storage
Store undiluted at 4°C.
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Species testing during development may have been performed with a different format of the same clone. Selected applications have been tested for cross-reactivity.
- Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
This reagent is effective for indirect immunofluorescence staining of human tissue for flow cytometric analysis. For flow cytometric applications, a three step labelling procedure is recommended for amplifying signal.
Suggested protocol for 3-step staining using Lysed Whole Blood method:
1. Incubate 100 µl whole blood with primary (unconjugated) antibody for 20-30 minutes at room temperature.
2. Add 2 mls of 1X Pharm Lyse (10X Pharm Lyse, Cat. No. 555899) and incubate for 10-15 minutes. Centrifuge and aspirate.
3. Wash once with PBS/0.1% sodium azide/ 1% heat-inactivated fetal bovine serum (PBS-FBS). Centrifuge and aspirate.
4. Add biotinylated mouse anti-rat Ig's (Cat. No. 553883) and incubate for 20-30 minutes at room temperature.
5. Wash once with PBS-FBS. Centrifuge and aspirate.
6. Add SAV-PE (Cat. No. 554061) and incubate for 20-30 minutes in the dark at room temperature.
7. Wash once with PBS-FBS. Centrifuge and aspirate Resuspend in 0.5 ml of PBS-FBS and analyze by flow cytometry.