Description
The CMab-43 monoclonal antibody specifically recognizes Prominin-1, which is also known as Prominin (mouse)-like 1 (PROML1 or Prominin-like protein 1), hProminin, Hematopoietic stem cell antigen, Macular dystrophy retinal 2 (MCDR2), Stargardt disease 4 autosomal dominant (STGD4), AC133 antigen, or CD133. Prominin-1 (CD133) is a ~120 kDa five-transmembrane glycoprotein that is encoded by PROM1 (Prominin 1) which belongs to the Prominin gene family. This single-chain, pentaspan transmembrane glycoprotein is comprised of an extracellular N-terminus with two short intracellular sequences and two long extracellular loops followed by an intracellular C-terminus. Prominin-1 (CD133) is expressed on hematopoietic stem cells and progenitor cells found in human bone marrow, cord blood and peripheral blood. It is also expressed on neural stem cells, other tissue-specific stem cells, endothelial progenitor cells, epithelial cells during development, and retinal cells. Prominin-1 (CD133) is likewise detected on some cancer cells from solid tumors, leukemias, cell lines, and can serve as a marker for cancer stem cells. Mutations in PROM1 have reportedly been associated with some retinal diseases.
Format
Suggested Companion Products
Preparation and Storage
Store undiluted at 4°C.
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
