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PE Mouse anti-Human CD337 (NKp30)
PE Mouse anti-Human CD337 (NKp30)
Expression of CD337 (NKp30) in human NK cells.  Human blood cells were stained with either PE Mouse anti-Human CD337 (NKp30)(Cat. No. 558407; right panel) or PE mouse IgG1 isotype control (Cat. No. 554680; left panel) and Alexa Fluor 488 Mouse anti-Human CD56 (Cat. No. 557699).  Erythrocytes were lysed using BD PharmLyse™ lysis buffer (Cat. No. 555899).  Viable lymphocytes were selected by scatter profile.  Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system.
Expression of CD337 (NKp30) in human NK cells.  Human blood cells were stained with either PE Mouse anti-Human CD337 (NKp30)(Cat. No. 558407; right panel) or PE mouse IgG1 isotype control (Cat. No. 554680; left panel) and Alexa Fluor 488 Mouse anti-Human CD56 (Cat. No. 557699).  Erythrocytes were lysed using BD PharmLyse™ lysis buffer (Cat. No. 555899).  Viable lymphocytes were selected by scatter profile.  Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system.
Product Details
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BD Pharmingen™
NCR3; Natural cytotoxicity triggering receptor 3; NKp30; MALS; LY117; 1C7
Human (QC Testing)
Mouse IgG1, κ
Horse NKp30 extracellular domain
Flow cytometry (Routinely Tested)
0.2 mg/ml
259197
AB_647240
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  5. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
558407 Rev. 3
Antibody Details
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p30-15

The p30-15 monoclonal antibody specifically binds to CD337, also known as NKp30, a receptor found on the surface of natural killer (NK) cells.  NK cells are large lymphoid cells discovered because of their ability to recognize and kill abnormal cells such as tumor and virally infected cells.  NK cell immune responses are regulated by a balance of activating and inhibitory signals generated by cell surface receptors.  Inhibitory receptors recognize MHC class I molecules on normal cells producing a negative signal to the NK cell.  Loss of MHC class I expression in infected or transformed cells results in the loss of this negative signal leading to NK cell activation.  In concert with the loss of inhibitory signals, activation signals via NK receptors such as NKp30, NKp44, NKp46, NKG2D, and NKp80 mediate the activation of NK cells.  NKp30 cooperates with NKp46 and/or NKp44 in the induction of NK cell-mediated cytotoxicity against the majority of target cells.

558407 Rev. 3
Format Details
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PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
558407 Rev.3
Citations & References
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Development References (5)

  1. Augugliaro R, Parolini S, Castriconi , et al. Selective cross-talk among natural cytotoxicity receptors in human natural killer cells. Eur J Immunol. 2003; 33(5):1235-1241. (Biology). View Reference
  2. Byrd A, Hoffmann SC, Jarahian M, Momburg F, Watzl C. Expression analysis of the ligands for the Natural Killer cell receptors NKp30 and NKp44. PLoS ONE. 2007; 2(12):e1339. (Immunogen). View Reference
  3. Flaig RM, Stark S, Watzl C. Cutting edge: NTB-A activates NK cells via homophilic interaction. J Immunol. 2004; 172(11):6524-6527. (Biology). View Reference
  4. Pende D, Parolini S, Pessino A, et al. Identification and molecular characterization of NKp30, a novel triggering receptor involved in natural cytotoxicity mediated by human natural killer cells. J Exp Med. 1999; 190(10):1505-1516. (Biology). View Reference
  5. Stark S, Flaig RM, Sandusky M, Watzl C. The use of trimeric isoleucine-zipper fusion proteins to study surface-receptor-ligand interactions in natural killer cells. J Immunol Methods. 2004; 296(1-2):149-158. (Biology). View Reference
View All (5) View Less
558407 Rev. 3

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.