PE Mouse Anti-Human CD158b1, b2, j
Clone DX27 (RUO)
- Brand BD Pharmingen™
- Alternative Name NKAT2/NKAT5/NKAT6; KIR2DL3/KIR2DS2/KIR2DL2
- Vol. Per Test 20 µl
- Isotype Mouse IgG2a, κ
- Reactivity Human (QC Testing)
Flow cytometry (Routinely Tested)
- Entrez Gene ID 3804
- Storage Buffer Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
- Regulatory Status RUO
Regulatory Status Legend
The DX27 monoclonal antibody specifically recognizes CD158b1 (KIRDL2/NKAT6), CD158b2 (KIRDL3/NKAT2), and CD158j (KIR2DS2/NKAT5) which are members of the Killer immunoglobulin-like receptor (KIR) family within the Ig superfamily. CD158b1 and CD158j contain two (KIR2D) Ig-like extacellular domains whereas CD158b2 contains three (KIR3D) Ig-like domains. These polymorphic CD158 molecules are expressed on natural killer (NK) cells and a subset of T cells and can recognize MHC class I molecules on target cells. They serve as either inhibitory receptors (CD158b1, CD158b2) that express immunoreceptor tyrosine-based inhibitory motifs (ITIMs) in their cytoplasmic domains or activating receptors (CD158j) that lack ITIMs.
- Format PE
- Excitation Source Blue 488 nm,Green 532 nm,Yellow/Green 561 nm
- Excitation Max 496 nm
- Emission Max 578 nm
R-phycoerythrin (PE) is an accessory photosynthetic pigment found in red algae. It exists in vitro as a 240-kDa protein with 23 phycoerythrobilin chromophores per molecule. This makes PE one of the brightest fluorochromes for flow cytometry applications, but its photobleaching properties make it unsuitable for fluorescence microscopy.
Suggested Companion Products
|Resources & Tools|
|Spectrum Viewer||Panel Designer||Spectrum Viewer||Download TDS||Regulatory Document Website|
Preparation and Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- An isotype control should be used at the same concentration as the antibody of interest.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
BD™ CompBeads can be used as surrogates to assess fluorescence spillover (Compensation). When fluorochrome conjugated antibodies are bound to CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and CompBead to ensure that BD Comp beads are appropriate for your specific cellular application.