Multicolor flow cytometric analysis of Tspan-27 (CD82) expression on human peripheral blood lymphocytes. Whole blood was stained with BD Horizon™ BV421 Mouse Anti-Human CD3 [Cat. No.562426/562427; shown in Upper Panels] and PE Mouse Anti-Human CD56 (NCAM-1) [Cat. No. 563238; Lower Panels] antibodies and either Alexa Fluor® 647 Mouse IgG1, κ Isotype Control (Cat. No. 557714) [Left Panels] or Alexa Fluor® 647 Mouse Anti-Human Tspan-27 (CD82) antibody (Cat. No. 564341) [Right Panels]. The erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Two-color flow cytometric contour plots showing the correlated expression patterns of CD3 or CD56 versus Tspan-27 (CD82) (or Ig Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.
Multicolor flow cytometric analysis of Tspan-27 (CD82) expression on human peripheral blood lymphocytes. Whole blood was stained with BD Horizon™ BV421 Mouse Anti-Human CD3 [Cat. No.562426/562427; shown in Upper Panels] and PE Mouse Anti-Human CD56 (NCAM-1) [Cat. No. 563238; Lower Panels] antibodies and either Alexa Fluor® 647 Mouse IgG1, κ Isotype Control (Cat. No. 557714) [Left Panels] or Alexa Fluor® 647 Mouse Anti-Human Tspan-27 (CD82) antibody (Cat. No. 564341) [Right Panels]. The erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Two-color flow cytometric contour plots showing the correlated expression patterns of CD3 or CD56 versus Tspan-27 (CD82) (or Ig Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.
Product Details
BD Pharmingen™
KAI1; 4F9; C33; IA4; R2; SAR2; ST6; GR15; Tspan-27; Tetraspanin-27
Human (QC Testing)
Mouse IgG1, κ
Human CD82 Transfected Cell Line
Flow cytometry (Routinely Tested)
5 µl/test
AB_2738755
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.
Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
- Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
- Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- An isotype control should be used at the same concentration as the antibody of interest.
Companion Products
Stain Buffer (FBS) RUO
Size
500 mL
Cat No.
554656
Stain Buffer (BSA) RUO
Size
500 mL
Cat No.
554657
Lysing Buffer RUO
Size
100 mL
Cat No.
555899
Alexa Fluor® 647 Mouse IgG1 κ Isotype Control RUO
Size
100 Tests
Cat No.
557714
BV421 Mouse Anti-Human CD3 RUO
Size
100 Tests
Cat No.
562426
BV421 Mouse Anti-Human CD3 RUO
Size
25 Tests
Cat No.
562427
564341 Rev. 1
Antibody Details
423524
The 423524 monoclonal antibody specifically binds to Tetraspanin-27 (Tspan-27) which is also known as CD82, R2, C33, KAI1, 4F9, IA4, and ST6. The CD82 glycoprotein is a member of tetraspanin superfamily that has four putative transmembrane domains. CD82 is widely expressed on a variety of cell types including granulocytes, monocytes, fibroblasts, epithelial cells, endothelial cells, CD34+ progenitor cells, and at varying levels on T cell and B cell subsets. CD82 associates with other surface molecules including integrins, MHC molecules, CD37, CD53, and CD81 and forms signaling receptor complexes. These can regulate cellular responses including the activation of monocytes and costimulation of T cells. CD82 appears to play a role in the suppression of tumor cell metastasis.
564341 Rev. 1
Format Details
Alexa Fluor™ 647
Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 520-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Alexa Fluor™ 647
Red 627-640 nm
653 nm
669 nm
564341 Rev.1
Citations & References
Development References (6)
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Burchert A, Notter M, Dietrich Menssen H, et al. CD82 (KAI1), a member of the tetraspan family, is expressed on early haemopoietic progenitor cells and up-regulated in distinct human leukaemias. Br J Haematol. 1999; 107(3):494-507. (Biology). View Reference
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Lebel-Binay S, Lagaudriere C, Fradelizi D, Conjeaud H. CD82, member of the tetra-span-transmembrane protein family, is a costimulatory protein for T cell activation. J Immunol. 1995; 155(1):101-110. (Biology). View Reference
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Shibagaki N, Hanada K, Yamaguchi S, Yamashita H, Shimada S, Hamada H. Functional analysis of CD82 in the early phase of T cell activation: roles in cell adhesion and signal transduction. Eur J Immunol. 1998; 28(4):1125-1133. (Biology). View Reference
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Spring FA, Griffiths RE, Mankelow TJ, et al. Tetraspanins CD81 and CD82 facilitate alpha4beta1-mediated adhesion of human erythroblasts to vascular cell adhesion molecule-1. PLoS ONE. 2013; 8(5):1-15. (Biology). View Reference
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Tsai YC, Weissman AM. Dissecting the diverse functions of the metastasis suppressor CD82/KAI1. FEBS Lett. 2011; 585(20):3166-3173. (Biology). View Reference
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Zola H. Leukocyte and stromal cell molecules : the CD markers. Hoboken, N.J.: Wiley-Liss; 2007.
564341 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
