Preparation And Storage
Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- An isotype control should be used at the same concentration as the antibody of interest.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
- Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
- Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
The 586326 monoclonal antibody specifically recognizes Leukocyte Immunoglobulin-like Receptor 6 (LIR-6) which is also known as CD85 antigen-like family member I (CD85i). LIR-6 (CD85i) is a type I transmembrane glycoprotein that is encoded by LILRA1 (Leukocyte immunoglobulin like receptor A1) and belongs to the LILR family within the Ig gene superfamily. A LIR-6a isoform with four extracellular Ig-like domains and a LIR-6b isoform with two extracellular Ig-like domains have been reported. LIR-6 (CD85i) has a short cytoplasmic domain and a charged arginine residue within its transmembrane region which interacts with Fc Receptor gamma (FcR?) and allows it to serve as an activating receptor. It is expressed on monocytes and B cells and at lower levels on dendritic cells and NK cells. LIRs interact with HLA class I ligands and contribute to the regulation of immune responses. LIR-6 preferentially binds to HLA-C free heavy chains and to HLA-B27. The 586326 antibody crossreacts with human CD85j recombinant protein in a direct ELISA but does not crossreact with human CD85a, CD85c, CD85d, CD85e, CD85h, nor CD85k recombinant proteins.
Development References (3)
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Borges L, Hsu ML, Fanger N, Kubin M, Cosman D. A family of human lymphoid and myeloid Ig-like receptors, some of which bind to MHC class I molecules. J Immunol. 1997; 159(11):5192-5196. (Biology). View Reference
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Brown D, Trowsdale J, Allen R. The LILR family: modulators of innate and adaptive immune pathways in health and disease. Tissue Antigens. 2004; 64(3):215-225. (Biology). View Reference
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Lichterfeld M, Yu XG. The emerging role of leukocyte immunoglobulin-like receptors (LILRs) in HIV-1 infection. J Leukoc Biol. 2012; 91(1):27-33. (Biology). View Reference
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
