Alexa Fluor® 647 Mouse Anti-Human CRTAM (CD355)
Clone Cr24.1 (RUO)
- Brand BD Pharmingen™
- Alternative Name CRTAM; class-I MHC-restricted T-cell-associated molecule; cytotoxic and regulatory T-cell molecule
- Concentration 0.2 mg/ml
- Isotype Mouse BALB/c IgG2a, κ
- Reactivity Human (QC Testing)
Flow cytometry (Routinely Tested)
- Immunogen Human CRTAM Transfected Cell Line
- Storage Buffer Aqueous buffered solution containing ≤0.09% sodium azide.
- Regulatory Status RUO
Regulatory Status Legend
The CR24.1 monoclonal antibody specifically recognizes Class I MHC-restricted T cell-associated molecule (CRTAM) which is also known as CD355, Class I-restricted T cell-associated molecule, or Cytotoxic and regulatory T-cell molecule. CRTAM (CD355) is a type I transmembrane glycoprotein that belongs to the Nectin family of cell adhesion molecules within the Ig superfamily. It is expressed on activated NK cells, NKT cells, γδ T cells, CD8+ T cells, and by Purkinje neurons of the cerebellum. CRTAM can exist as a homodimer or as a heterodimer when bound to its ligand, Nectin-like molecule 2 (Necl-2), which is encoded by TSLC1 (tumor suppressor gene tumor suppressor in lung cancer-1). Necl-2-CRTAM interactions play important roles in cellular adhesion and migration and can augment IFN-γ production by CD8+ T cells and cytotoxicity by NK cells.
Alexa Fluor® 647 conjugates are highly photostable and remain fluorescent over a broad pH range. The excitation and emission maxima are nearly identical to those of APC. However, APC tends to be brighter while Alexa Fluor® 647 is more optimal for intracellular applications. This fluorochrome exhibits uncommon photostability, making it an ideal choice for use in fluorescence microscopy. Due to nearly identical excitation and emission properties but different spillover characteristics, APC and Alexa Fluor® 647 cannot be used simultaneously.
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Preparation and Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
- Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
- Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.