Flow cytometric analysis of human CD167a expression on T47D cells. Cells from the human T-47D (Breast carcinoma ATCC HTB133) cell line were stained with either with Alexa Fluor® 647 Mouse IgM, κ Isotype Control (Cat. No. 560806; dashed line histogram) or Alexa Fluor® 647 Mouse Anti-Human CD167a antibody (Cat. No. 566236; solid line histogram). The fluorescence histogram showing CD167a expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analysis was performed using a BD FACSCanto™ II Flow Cytometer System.
Immunohistofluorescent staining of Human CD167a in human breast cancer. Following antigen retrieval with BD Pharmingen™ Retrievagen A Buffer (Cat. No. 550524), a section from formalin-fixed, paraffin-embedded human breast cancer tissue was stained with Alexa Fluor® 647 Mouse Anti-Human CD167a antibody (Cat. No. 566236; pseudocolored green) and DAPI Solution (Cat. No. 564907; pseudocolored red). The images were analyzed and merged using a Molecular Devices Epifluorescence Microscopy System. Original magnification, 20x.
Flow cytometric analysis of human CD167a expression on T47D cells. Cells from the human T-47D (Breast carcinoma ATCC HTB133) cell line were stained with either with Alexa Fluor® 647 Mouse IgM, κ Isotype Control (Cat. No. 560806; dashed line histogram) or Alexa Fluor® 647 Mouse Anti-Human CD167a antibody (Cat. No. 566236; solid line histogram). The fluorescence histogram showing CD167a expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analysis was performed using a BD FACSCanto™ II Flow Cytometer System.
Immunohistofluorescent staining of Human CD167a in human breast cancer. Following antigen retrieval with BD Pharmingen™ Retrievagen A Buffer (Cat. No. 550524), a section from formalin-fixed, paraffin-embedded human breast cancer tissue was stained with Alexa Fluor® 647 Mouse Anti-Human CD167a antibody (Cat. No. 566236; pseudocolored green) and DAPI Solution (Cat. No. 564907; pseudocolored red). The images were analyzed and merged using a Molecular Devices Epifluorescence Microscopy System. Original magnification, 20x.
Product Details
BD Pharmingen™
CD167; CAK; DDR; EDDR1; HGK2; MCK10; NEP; NTRK4; PTK3; PTK3A; RTK6; TRKE
Human (QC Testing)
Mouse BALB/c IgM, κ
Human DDR1 Transfected Cell Line
Flow cytometry (Routinely Tested), Immunofluorescence (Tested During Development)
0.2 mg/ml
VII 70263
AB_2739621
Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
- Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
- Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
Stain Buffer (FBS) RUO
Size
500 mL
Cat No.
554656
Stain Buffer (BSA) RUO
Size
500 mL
Cat No.
554657
Retrievagen A (pH 6.0) RUO
Cat No.
550524
Alexa Fluor® 647 Mouse IgM, κ Isotype Control RUO
Size
0.1 mg
Cat No.
560806
DAPI Solution RUO
Size
1 mg
Cat No.
564907
566236 Rev. 1
Antibody Details
48B3
The 48B3 monoclonal antibody specifically binds to CD167a which is also known as Discoidin domain receptor tyrosine kinase 1 (DDR1), Cell adhesion kinase (CAK), Mammary carcinoma kinase 10 (MCK10), or Neuroepithelial tyrosine kinase. CD167a is expressed on epithelial cells and at relatively low levels on immature dendritic cells and B lymphocytes. CD167a is a single-pass type I transmembrane glycoprotein. The extracellular CD167a discoidin homology domain mediates binding to various types of collagen. The binding of collagen leads to the activation of the cytoplasmic tyrosine kinase domain. The CD167a receptor tyrosine kinase (RTK) plays a role in regulating cellular proliferation, morphogenesis, differentiation, and collagen synthesis, as well as the synthesis of metalloproteases and extracellular matrix remodeling. CD167a may be involved in tumor invasion and metastasis. The 48B3 antibody does not recognize CD167b (DDR2).
566236 Rev. 1
Format Details
Alexa Fluor™ 647
Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 520-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Alexa Fluor™ 647
Red 627-640 nm
653 nm
669 nm
566236 Rev.1
Citations & References
Development References (4)
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Bühring HJ, Buckley CD, Simmins DL. Review—CD167a—DDR1; discoidin domain receptor 1. In: Mason D. David Mason .. et al., ed. Leucocyte typing VII : white cell differentiation antigens : proceedings of the Seventh International Workshop and Conference held in Harrogate, United Kingdom. Oxford: Oxford University Press; 2002:21.
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Johnson JD, Edman JC, Rutter WJ. A receptor tyrosine kinase found in breast carcinoma cells has an extracellular discoidin I-like domain.. Proc Natl Acad Sci USA. 1993; 90(22):10891. (Biology). View Reference
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Vogel W, Gish GD, Alves F, Pawson T. The discoidin domain receptor tyrosine kinases are activated by collagen.. Mol Cell. 1997; 1(1):13-23. (Biology). View Reference
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Zola H, Swart B, Boumsell L, Mason DY. Human Leucocyte Differentiation Antigen nomenclature: update on CD nomenclature. Report of IUIS/WHO Subcommittee.. J Immunol Methods. 2003; 275(1-2):1-8. (Clone-specific: Flow cytometry). View Reference
566236 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
