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Alexa Fluor® 488 Mouse Anti-Human CD199 (CCR9)
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Alexa Fluor® 488 Mouse Anti-Human CD199 (CCR9)
Flow cytometric analysis of CD199 (CCR9) expressed on MOLT-4 cells. Human MOLT-4 cells were stained with either Alexa Fluor® 488 Mouse Anti-Human CD199 (CCR9) antibody (Cat. No. 561608, solid line histogram) or an Alexa Fluor® 488 mIgG2a, κ Isotype Control (Cat. No. 557703; dashed line histogram). Flow cytometric fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometry was performed using a BD LSR™ II Flow Cytometer System.
Flow cytometric analysis of CD199 (CCR9) expressed on MOLT-4 cells. Human MOLT-4 cells were stained with either Alexa Fluor® 488 Mouse Anti-Human CD199 (CCR9) antibody (Cat. No. 561608, solid line histogram) or an Alexa Fluor® 488 mIgG2a, κ Isotype Control (Cat. No. 557703; dashed line histogram). Flow cytometric fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometry was performed using a BD LSR™ II Flow Cytometer System.
Product Details
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BD Pharmingen™
CDw199; Chemokine (C-C motif) receptor 9; GPR-9-6; GPR28
Human (QC Testing)
Mouse IgG2a
Human CCR9 Transfected Cell Line
Flow cytometry (Routinely Tested)
0.2 mg/ml
VIII 80134
AB_10898012
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 488 under optimum conditions, and unreacted Alexa Fluor® 488 was removed.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
  5. Alexa Fluor® 488 fluorochrome emission is collected at the same instrument settings as for fluorescein isothiocyanate (FITC).
  6. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
  7. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  8. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
561608 Rev. 2
Antibody Details
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112509

The 112509 monoclonal antibody specifically binds to the human CCR9 chemokine receptor. CCR9 is a thymus specific CC chemokine receptor expressed at high levels by essentially all CD4+ and CD8+ T lymphocytes. CCR9 and its ligand CCL25/TECK are involved in lymphocyte recruitment in the small intestine. The immunogen used to generate the 112509 hybridoma was human CCR9-transfected cells.

561608 Rev. 2
Format Details
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Alexa Fluor™ 488
Alexa Fluor™ 488 Dye is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 517-nm. Alexa Fluor™ 488 is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 520-nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Alexa Fluor™ 488
Blue 488 nm
494 nm
517 nm
561608 Rev.2
Citations & References
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Development References (4)

  1. Kunkel EJ, Campbell JJ, Haraldsen G, et al. Lymphocyte CC chemokine receptor 9 and epithelial thymus-expressed chemokine (TECK) expression distinguish the small intestinal immune compartment: Epithelial expression of tissue-specific chemokines as an organizing principle in regional immunity. J Exp Med. 2000; 192(5):761-768. (Biology). View Reference
  2. Kunkel EJ, Kim CH, Lazarus NH, et al. CCR10 expression is a common feature of circulating and mucosal epithelial tissue IgA Ab-secreting cells.. J Clin Invest. 2003; 111(7):1001-1010. (Biology). View Reference
  3. Zaballos A, Gutierrez J, Varona R, Ardavin C, Marquez G. Cutting edge: identification of the orphan chemokine receptor GPR-9-6 as CCR9, the receptor for the chemokine TECK. J Immunol. 1999; 162(10):5671-5675. (Biology). View Reference
  4. Zabel BA, Agace WW, Campbell JJ, et al. Human G protein-coupled receptor GPR-9-6/CC chemokine receptor 9 is selectively expressed on intestinal homing T lymphocytes, mucosal lymphocytes, and thymocytes and is required for thymus-expressed chemokine-mediated chemotaxis.. J Exp Med. 1999; 190(9):1241-1256. (Biology). View Reference
View All (4) View Less
561608 Rev. 2

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.