Multicolor flow cytometric analysis CD160 expression on human peripheral blood lymphocytes. Human whole blood cells were stained with PE-Cy™7 Mouse Anti-Human CD56 (Cat. No. 557747/560916) and either Alexa Fluor® 488 Mouse IgM, κ Isotype Control (Cat. No. 562409; Left Panel) or Alexa Fluor® 488 Mouse anti-Human CD160 (Cat. No. 562351; Right Panel). The erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Two-color flow cytometric dot plots showing the correlated expression patterns of CD160 (or Ig isotype control staining) versus CD56 were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.
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Multicolor flow cytometric analysis CD160 expression on human peripheral blood lymphocytes. Human whole blood cells were stained with PE-Cy™7 Mouse Anti-Human CD56 (Cat. No. 557747/560916) and either Alexa Fluor® 488 Mouse IgM, κ Isotype Control (Cat. No. 562409; Left Panel) or Alexa Fluor® 488 Mouse anti-Human CD160 (Cat. No. 562351; Right Panel). The erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Two-color flow cytometric dot plots showing the correlated expression patterns of CD160 (or Ig isotype control staining) versus CD56 were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.
Product Details
BD Pharmingen™
BY55; BY55 antigen; NK1; NK28
Human (QC Testing)
Mouse BALB/c IgM
Human YT2C2 NK Cell Line
Flow cytometry (Routinely Tested)
5 µl
VII
11126
AB_11153688
Aqueous buffered solution containing BSA, protein stabilizer, and ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 488 under optimum conditions, and unreacted Alexa Fluor® 488 was removed.
Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- An isotype control should be used at the same concentration as the antibody of interest.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
- Alexa Fluor® 488 fluorochrome emission is collected at the same instrument settings as for fluorescein isothiocyanate (FITC).
- Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
Stain Buffer (FBS) RUO
Size
500 mL
Cat No.
554656
Lysing Buffer RUO
Size
100 mL
Cat No.
555899
Alexa Fluor® 488 Mouse IgM, κ Isotype Control RUO
Size
0.1 mg
Cat No.
562409
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PE-Cy™7 Mouse Anti-Human CD56 (NCAM-1) RUO
Size
25 Tests
Cat No.
560916
PE-Cy™7 Mouse Anti-Human CD56 (NCAM-1) RUO
Size
100 Tests
Cat No.
557747
562351 Rev. 1
Antibody Details
BY55
The BY55 monoclonal antibody specifically binds to the human cell surface molecule, CD160 (also known as BY55). CD160 is a glycosyolphosphatidylinositol-anchored glycoprotein member of the immunoglobulin superfamily. It is expressed on cytotoxic αβ CD8+ T cells and natural killer (NK) cells, γδ T cells and intestinal intraepithelial T lymphocytes. CD160 can bind to classical and nonclassical MHC class I molecules. This interaction can costimulate the proliferation of activated human T lymphocytes including CD28-negative T lymphocytes.
562351 Rev. 1
Format Details
Alexa Fluor™ 488
Alexa Fluor™ 488 Dye is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 517-nm. Alexa Fluor™ 488 is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 520-nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Alexa Fluor™ 488
Blue 488 nm
494 nm
517 nm
562351 Rev.1
Citations & References
Development References (3)
-
Agrawal S, Marquet J, Freeman GJ, et al. Cutting edge: MHC class I triggering by a novel cell surface ligand costimulates proliferation of activated human T cells. J Immunol. 1999; 162(3):1223-1226. (Clone-specific: Flow cytometry). View Reference
-
Anumanthan A, Bensussan A, Boumsell L, et al. Cloning of BY55, a novel Ig superfamily member expressed on NK cells, CTL, and intestinal intraepithelial lymphocytes. J Immunol. 1998; 161(6):2780-2790. (Clone-specific: Flow cytometry, Immunoprecipitation). View Reference
-
Maiza H, Leca G, Mansur IG, Schiavon V, Boumsell L, Bensussan A. A novel 80-kD cell surface structure identifies human circulating lymphocytes with natural killer activity. J Exp Med. 1993; 178(3):1121-1126. (Clone-specific: Flow cytometry). View Reference
562351 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
