Description
Cell or tissue lysates are a useful control for the western blotting application. A source that can be rich in endogenously expressed proteins, this lysate is convenient to use and provided in a denatured format allowing for immediate loading onto an SDS-PAGE gel.
This lysate was routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
Preparation And Storage
NIH 3T3 cells were serum starved overnight and then incubated at room temperature for 10 minutes in a final concentration of 1 mM sodium vanadate. PDGF was then added at a final concentration of 5 ng/ml and cells were incubated at 37°C for 30 minutes. Western blots of NIH 3T3 cell lysates treated with vanadate and PDGF have been reported to show a shift in SHC mobility in addition to phosphorylation of PKBα/Akt and Stat3 as compared to untreated control cells.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
