Multiparameter flow cytometric analysis of human STING expression in human peripheral blood leukocytes. Human blood was treated with BD Phosflow™ Lyse/Fix Buffer (Cat. No. 558049) followed by permeablization with BD Perm/Wash™ Buffer (Cat. No. 554723). Cells were then stained with BD Horizon™ BV421 Mouse Anti-Human CD20 antibody (Cat. No. 563346) and either Alexa Fluor® 647 IgG1, κ Isotype Control (Cat. No. 565571, Top Panels) or Alexa Fluor®647 Mouse Anti-Human STING antibody (Cat. No. 564836; Bottom Panels). Left Panels - Contour plots showing the correlated expression of STING (or Ig Isotype control staining) versus Side Light Scatter (SSC) were derived from gated events with the forward and side light-scatter characteristics of intact leucocytes. Right Panels - Contour plots showing the correlated expression of STING (or Ig Isotype control staining) versus CD20 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD FACSCanto™ II Flow Cytometer System.
Multiparameter flow cytometric analysis of human STING expression in human peripheral blood leukocytes. Human blood was treated with BD Phosflow™ Lyse/Fix Buffer (Cat. No. 558049) followed by permeablization with BD Perm/Wash™ Buffer (Cat. No. 554723). Cells were then stained with BD Horizon™ BV421 Mouse Anti-Human CD20 antibody (Cat. No. 563346) and either Alexa Fluor® 647 IgG1, κ Isotype Control (Cat. No. 565571, Top Panels) or Alexa Fluor®647 Mouse Anti-Human STING antibody (Cat. No. 564836; Bottom Panels). Left Panels - Contour plots showing the correlated expression of STING (or Ig Isotype control staining) versus Side Light Scatter (SSC) were derived from gated events with the forward and side light-scatter characteristics of intact leucocytes. Right Panels - Contour plots showing the correlated expression of STING (or Ig Isotype control staining) versus CD20 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD FACSCanto™ II Flow Cytometer System.
Product Details
BD Pharmingen™
hSTING; MITA; hMITA; ERIS; MPYS; NET23; TM173; TMEM173
Human (QC Testing), Mouse (Lack of Reactivity Confirmed in Development)
Mouse IgG1, κ
Human STING Recombinant Protein
Intracellular staining (flow cytometry) (Routinely Tested)
5 µl/test
AB_2738974
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.
Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- An isotype control should be used at the same concentration as the antibody of interest.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
- Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
- Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
- Species cross-reactivity detected in product development may not have been confirmed on every format and/or application.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- This product is provided under an intellectual property license between Life Technologies Corporation and BD Businesses. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for Commercial Purposes. Commercial Purposes means any activity by a party for consideration and may include, but is not limited to: (1) use of the product or its components in manufacturing; (2) use of the product or its components to provide a service, information, or data; (3) use of the product or its components for therapeutic, diagnostic or prophylactic purposes; or (4) resale of the product or its components, whether or not such product or its components are resold for use in research. For information on purchasing a license to this product for any other use, contact Life Technologies Corporation, Cell Analysis Business Unit Business Development, 29851 Willow Creek Road, Eugene, OR 97402, USA, Tel: (541) 465-8300. Fax: (541) 335-0504.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
Stain Buffer (FBS) RUO
Size
500 mL
Cat No.
554656
Lyse/Fix Buffer 5X RUO
Size
250 mL
Cat No.
558049
Perm/Wash Buffer RUO
Size
100 mL
Cat No.
554723
BV421 Mouse Anti-Human CD20 RUO
Size
50 Tests
Cat No.
563346
564836 Rev. 4
Antibody Details
T3-680
The T3-680 monoclonal antibody specifically recognizes human STING (Stimulator of interferon genes protein) which is also known as Endoplasmic reticulum interferon stimulator (ERIS) and Mediator of IRF3 activation (MITA). STING is encoded by TMEM173 (Transmembrane protein 173). STING is a multi-pass transmembrane adaptor protein which is widely expressed in the endoplasmic reticulum of cells. STING acts as a sensor of cytosolic double-stranded DNA derived from bacteria and viruses and promotes the production of type I interferons, IFN-α and IFN-β. Following activation, STING translocates with TBK1 kinase to perinuclear endosomes. The TBK1 kinase phosphorylates and activates interferon regulatory factors, such as IRF3, and NF-κB, which leads to the induction of type I interferon and other immune response genes that play roles in innate and adaptive immunity.
564836 Rev. 4
Format Details
Alexa Fluor™ 647
Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 520-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Alexa Fluor™ 647
Red 627-640 nm
653 nm
669 nm
564836 Rev.4
Citations & References
Development References (5)
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Ablasser A, Goldeck M, Cavlar T, et al. cGAS produces a 2'-5'-linked cyclic dinucleotide second messenger that activates STING. Nature. 2013; 498(7454):380-384. (Biology). View Reference
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Ishikawa H, Barber GN. STING is an endoplasmic reticulum adaptor that facilitates innate immune signalling. Nature. 2008; 455(7213):674-678. (Biology). View Reference
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Sun L, Wu J, Du F, Chen X, Chen ZJ. Cyclic GMP-AMP synthase is a cytosolic DNA sensor that activates the type I interferon pathway. Science. 2012; 339(6121):786-791. (Biology). View Reference
-
Unterholzner L, Keating SE, Baran M, et al. IFI16 is an innate immune sensor for intracellular DNA. Nat Immunol. 2010; 11(11):997-1004. (Biology). View Reference
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Zhang Z, Yuan B, Bao M, Lu N, Kim T, Liu YJ. The helicase DDX41 senses intracellular DNA mediated by the adaptor STING in dendritic cells. Nat Immunol. 2012; 12(10):959-965. (Biology). View Reference
564836 Rev. 4
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
