Clone SK3 (also known as Leu3a) (ASR)
- Brand BD Horizon™
- Concentration 6.3 µg/mL
- Vol. Per Test 5 µL
- Isotype Mouse BALB/c IgG1, κ
- Reactivity Human (QC Testing)
Flow cytometry (Routinely Tested)
- Immunogen Human Peripheral Blood T Cells
- Workshop No. I T38; III T140,496
- Entrez Gene ID 920
- Storage Buffer Phosphate buffered saline containing BSA and ProClin® 950
- Regulatory Status ASR
Regulatory Status Legend
The CD4 antibody, clone SK3, is derived from the hybridization of NS-1 mouse myeloma cells with spleen cells isolated from BALB/c mice immunized with human peripheral blood T lymphocytes.
The CD4 antibody recognizes a 55-kilodalton (kDa) glycoprotein that is present on T-helper/inducer lymphocytes and monocytes.
BV510 is a polymer-based dye that is brighter than BD Horizon V500. Due to similar excitation and emission properties, BD Horizon BV510 and BD Horizon V500 cannot be used simultaneously.
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Preparation and Storage
Store vials at 2°C–8°C. Conjugated forms should not be frozen. Protect from exposure to light. Each reagent is stable until the expiration date shown on the bottle label when stored as directed.
Protection of Laboratory Workers from Occupationally Acquired Infections–Third Edition; ApprovedGuideline. Wayne, PA: Clinical and Laboratory Standards Institute; 2005. M29-A3.
Bernard A, Boumsell L, Hill C. Joint report of the first international workshop on human leucocytedifferentiation antigens by the investigators of the participating laboratories. In: Bernard A, Boumsell L,Dausset J, Milstein C, Schlossman SF, eds. Leucocyte Typing. New York, NY: Springer-Verlag;1984:9-108.
Centers for Disease Control. Update: universal precautions for prevention of transmission of humanimmunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in health-care settings.MMWR. 1988:37:377-388.
Engleman EG, Benike CJ, Glickman E, Evans RL. Antibodies to membrane structures that distinguishsuppressor/cytotoxic and helper T lymphocyte subpopulations block the mixed leukocyte reaction inman. J Exp Med. 1981;153:193-198.
Evans RL, Wall DW, Platsoucas CD, et al. Thymus-dependent membrane antigens in man: inhibition ofcell-mediated lympholysis by monoclonal antibodies to TH2 antigen. Proc Natl Acad Sci U S A.1981;78:544-548.
Kotzin BL, Benike CJ, Engleman EG. Induction of immunoglobulin-secreting cells in the allogeneic mixedleukocyte reaction: regulation by helper and suppressor lymphocyte subsets in man. J Immunol.1981;127:931-935.
Ledbetter JA, Evans RL, Lipinski M, Cunningham-Rundles C, Good RA, Herzenberg LA. Evolutionaryconservation of surface molecules that distinguish T lymphocyte helper/inducer and cytotoxic/suppressorsubpopulations in mouse and man. J Exp Med. 1981;153:310-323.
Reichert T, DeBruyère M, Deneys V, et al. Lymphocyte subset reference ranges in adult Caucasians. ClinImmunol Immunopathol. 1991;60:190-208.
Wood GS, Warner NL, Warnke RA. Anti–Leu-3/T4 antibodies react with cells of monocyte/macrophageand Langerhans lineage. J Immunol. 1983;131:212-216.