CD4 BV510

BD Horizon™ CD4 BV510

Name: CD4 BV510
Brand: BD Horizon™
SKU: 659454

Clone SK3 (also known as Leu3a)

(ASR)

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Product Details

Brand: BD Horizon™

Alternative Name: L3T4; Leu3a; T-cell surface antigen T4/Leu-3 ; W3/25 ; CD4 antigen (p55)

Reactivity: Human

Isotype: Mouse BALB/c IgG1, κ

Immunogen: Human Peripheral Blood T Cells

Application: Flow cytometry

Concentration: 6.3 µg/mL

Vol. Per Test: 5 μL

Workshop Number: I T38; III T140,496

Entrez Gene ID: 920

Regulatory Status: ASR

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Antibody Details

SK3

The CD4 antibody, clone SK3, is derived from the hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immunized with human peripheral blood T lymphocytes.

The CD4 antibody recognizes a 55-kilodalton (kDa) glycoprotein that is present on T-helper/inducer lymphocytes and monocytes.

659454 Rev. 1

Format Details

BV510

The BD Horizon Brilliant Violet™ 510 (BV510) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye with an excitation maximum (Ex Max) at 327-nm / 405-nm and an emission maximum (Em Max) at 512-nm. BV510, driven by BD innovation, is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 510-nm (e.g., a 525/50 bandpass filter). The dye can be excited by the UV (355-nm) laser resulting in cross-laser excitation and spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: BV510

Excitation Source: Violet 405 nm

Excitation Max: 327 nm, 405 nm

Emission Max: 512 nm

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Citations & References

Development References (9)

Bernard A, Boumsell L, Hill C. Joint report of the first international workshop on human leucocyte differentiation antigens by the investigators of the participating laboratories. In: Bernard A, Boumsell L, Dausset J, Milstein C, Schlossman SF, ed. Leucocyte Typing. New York, NY: Springer-Verlag; 1984:9-108.
Centers for Disease Control. Update: universal precautions for prevention of transmission of humanimmunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in health-care settings. MMWR. 1988; 37:377-388. (Biology).
Engleman EG, Benike CJ, Glickman E, Evans RL. Antibodies to membrane structures that distinguishsuppressor/cytotoxic and helper T lymphocyte subpopulations block the mixed leukocyte reaction inman. J Exp Med. 1981; 153:193-198. (Biology).
Evans RL, Wall DW, Platsoucas CD, et al. Thymus-dependent membrane antigens in man: inhibition of cell-mediated lympholysis by monoclonal antibodies to TH2 antigen. Proc Natl Acad Sci U S A. 1981; 78(1):544-548. (Biology). View Reference
Kotzin BL, Benike CJ, Engleman EG. Induction of immunoglobulin-secreting cells in the allogeneic mixed leukocyte reaction: regulation by helper and suppressor lymphocyte subsets in man. J Immunol. 1981; 127(9):931-935. (Biology). View Reference
Ledbetter JA, Evans RL, Lipinski M, Cunningham-Rundles C, Good RA, Herzenberg LA. Evolutionary conservation of surface molecules that distinguish T lymphocyte helper/inducer and cytotoxic/suppressor subpopulations in mouse and man. J Exp Med. 1981; 153(2):310-323. (Biology). View Reference
Protection of Laboratory Workers from Occupationally Acquired Infections. Approved Guideline. 2005; M29-A3. (Biology).
Reichert T, DeBruyere M, Deneys V, et al. Lymphocyte subset reference ranges in adult Caucasians. Clin Immunol Immunopathol. 1991; 60(2):190-208. (Biology). View Reference
Wood GS, Warner NL, Warnke RA. Anti- Leu-3/T4 antibodies react with cells of monocyte/macrophageand Langerhans lineage. J Immunol. 1983; 131:212-216. (Biology).

View All (9)

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