Clone SK3 (also known as Leu3a) (ASR)
- Brand BD Horizon™
- Concentration 6.3 μg/mL
- Vol. Per Test 5 μL
- Isotype Mouse BALB/c IgG1, κ
- Reactivity Human (QC Testing)
Flow cytometry (Routinely Tested)
- Immunogen Human Peripheral Blood T Cells
- Workshop No. I T38; III T140,496
- Entrez Gene ID 920
- Storage Buffer Phosphate buffered saline with BSA and ProClin® 950.
- Regulatory Status ASR
Regulatory Status Legend
The CD4 antibody, clone SK3, is derived from the hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immunized with human peripheral blood T lymphocytes.
The CD4 antibody recognizes a 55-kilodalton (kDa) glycoprotein that is present on T-helper/inducer lymphocytes and monocytes.
BV421 is a polymer-based dye excited by the violet laser and is one of the brightest fluorochromes offered by BD Biosciences. Conjugates are typically 10 times brighter than Pacific Blue™ conjugates and are often as bright as or brighter than PE conjugates. Due to nearly identical excitation and emission properties but different spillover characteristics, BD Horizon BV421, Pacific Blue™, and BD Horizon™ V450 cannot be used simultaneously.
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Preparation and Storage
Store vials at 2°C to 8°C. Conjugated forms should not be frozen. Protect from exposure to light. Each reagent is stable until the expiration date shown on the bottle label when stored as directed.
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Bernard A, Boumsell L, Hill C. Joint report of the first international workshop on human leucocytedifferentiation antigens by the investigators of the participating laboratories. In: Bernard A, Boumsell L,Dausset J, Milstein C, Schlossman SF, eds. Leucocyte Typing. New York, NY: Springer-Verlag;1984:9-108.
Centers for Disease Control. Update: universal precautions for prevention of transmission of humanimmunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in health-care settings.MMWR. 1988:37:377-388.
Engleman EG, Benike CJ, Glickman E, Evans RL. Antibodies to membrane structures that distinguishsuppressor/cytotoxic and helper T lymphocyte subpopulations block the mixed leukocyte reaction inman. J Exp Med. 1981;153:193-198.
Evans RL, Wall DW, Platsoucas CD, et al. Thymus-dependent membrane antigens in man: inhibition ofcell-mediated lympholysis by monoclonal antibodies to TH2 antigen. Proc Natl Acad Sci U S A.1981;78:544-548.
Kotzin BL, Benike CJ, Engleman EG. Induction of immunoglobulin-secreting cells in the allogeneic mixedleukocyte reaction: regulation by helper and suppressor lymphocyte subsets in man. J Immunol.1981;127:931-935.
Ledbetter JA, Evans RL, Lipinski M, Cunningham-Rundles C, Good RA, Herzenberg LA. Evolutionaryconservation of surface molecules that distinguish T lymphocyte helper/inducer and cytotoxic/suppressorsubpopulations in mouse and man. J Exp Med. 1981;153:310-323.
Reichert T, DeBruyère M, Deneys V, et al. Lymphocyte subset reference ranges in adult Caucasians. ClinImmunol Immunopathol. 1991;60:190-208.
Wood GS, Warner NL, Warnke RA. Anti–Leu-3/T4 antibodies react with cells of monocyte/macrophageand Langerhans lineage. J Immunol. 1983;131:212-216.