CD33 BV421

BD Horizon™ CD33 BV421

Name: CD33 BV421
Brand: BD Horizon™
SKU: 663980

Clone P67.6

(ASR)

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Product Details

Brand: BD Horizon™

Alternative Name: Siglec-3; SIGLEC3; Sialic acid-binding Ig-like lectin 3; p67; gp67; My9

Reactivity: Human

Isotype: Mouse BALB/c IgG1, κ

Immunogen: CD33-transfected FMY9S5 Cells

Application: Flow cytometry

Concentration: 25 μg/mL

Vol. Per Test: 5 μL

Workshop Number: IV M503

Entrez Gene ID: 945

Regulatory Status: ASR

Product Notices

Caution: If you choose to combine two or more BD Horizon Brilliant™ Dyes in the same multicolor staining cocktail, fluorescent dye interactions may cause staining artifacts without the use of a buffering solution, such as the BD Horizon™ Brilliant Stain Buffer.

663980 Rev. 1

Antibody Details

P67.6

The CD33 antibody, clone P67-6, is derived from the hybridization of Sp2/0 mouse myeloma cells with spleen cells isolated from BALB/c mice immunized with FMY9S5 cells containing the CD33 gene.

The CD33 antibody recognizes a human myelomonocytic antigen, with a molecular weight of 67 kilodaltons (kDa).

663980 Rev. 1

Format Details

BV421

The BD Horizon Brilliant Violet™ 421 (BV421) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye has an excitation maximum (Ex Max) of 407-nm and an emission maximum (Em Max) at 423-nm. Driven by BD innovation, BV421 is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 420-nm (e.g., a 431/28-nm or 450/50-nm bandpass filter). BV421 is an ideal alternative for V450 as it is approximately ten times brighter with less spillover into the BV510/V500 detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: BV421

Excitation Source: Violet 405 nm

Excitation Max: 407 nm

Emission Max: 423 nm

663980 Rev.1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

Analyte Specific Reagent. Analytical and performance characteristics are not established.