Anti-TCRγ/δ-1 APC-R700

BD Horizon™ Anti-TCRγ/δ-1 APC-R700

Name: Anti-TCRγ/δ-1 APC-R700
Brand: BD Horizon™
SKU: 657707

Clone 11F2

(ASR)

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Product Details

Brand: BD Horizon™

Alternative Name: TCRgd; γδ TCR; TRG@/TRD@; TCRG/TCRD; TCR gamma delta

Reactivity: Human

Isotype: Mouse BALB/c IgG1

Immunogen: Sepharose® bead/CD3/γ/δ TCR complex

Application: Flow cytometry

Concentration: 50 μg/mL

Vol. Per Test: 5 μL

Entrez Gene ID: 6964, 6965

Regulatory Status: ASR

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Antibody Details

11F2

The Anti–TCRγ/δ-1 antibody, clone 11F2, is derived from hybridization of mouse Sp2/0 myeloma cells with spleen cells from BALB/c mice immunized with a Sepharose® bead/CD3/γ/δ TCR complex.

The Anti–TCRγ/δ-1 antibody reacts with a framework epitope of the γ/δ T-cell antigen receptor (TCR). The γ/δ TCR is a heterodimeric glycoprotein that is noncovalently associated with the CD3 antigen. The γ and δ TCR chains are composed of constant and variable regions, each encoded by distinct gene segments. The γ chain forms either disulfide-linked or non–disulfide-linked heterodimers with the δ-subunit.

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Format Details

APC-R700

The BD Horizon™ APC-R700 (APC-R700) Dye is a part of the BD APC red family of dyes. This tandem fluorochrome is comprised of an Allophycocyanin (APC) dye donor that has excitation maximum (Ex Max) of 651-nm and an acceptor dye, R700, with an emission maximum (Em Max) at 706-nm. APC-R700, driven by BD innovation, is designed to be excited by the red (627–640-nm) laser and detected using an optical filter centered near 710-nm (e.g., a 720/40-nm bandpass filter). APC-R700 is a brighter alternative to Alexa Fluor™ 700. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: APC-R700

Excitation Source: Red 627-640 nm

Excitation Max: 651 nm

Emission Max: 706 nm

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Citations & References

Development References (8)

Borst J, van Dongen JJ, Bolhuis RL, et al. Distinct molecular forms of human T cell receptor gamma/delta detected on viable T cells by a monoclonal antibody.. J Exp Med. 1988; 167(5):1625-44. (Biology). View Reference
Centers for Disease Control. Update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in healthcare settings. MMWR. 1988; 37:377-388. (Biology).
Clinical and Laboratory Standards Institute. 2005. (Biology).
Ichinohasama R, Miura I, Takahashi T, et al. Peripheral CD4+ CD8- gammadelta T cell lymphoma: a case report with multiparameter analyses.. Hum Pathol. 1996; 27(12):1370-7. (Biology). View Reference
Lanier L, Federspiel N, Ruitenberg J, et al. The T cell antigen receptor complex expressed on normal peripheral blood CD4-, CD8-T lymphocytes. J Exp Med. 1987; 165:1076-1094. (Biology).
Lanier LL, Ruitenberg J, Bolhuis RL, Borst J, Phillips JH, Testi R. Structural and serological heterogeneity of gamma/delta T cell antigen receptor expression in thymus and peripheral blood.. Eur J Immunol. 1988; 18(12):1985-92. (Biology). View Reference
Lanier LL, Serafini AT, Ruitenberg JJ, et al. The γ T-cell antigen receptor. J Clin Immunol. 1987; 7:429-440. (Biology).
Testi R, Lanier LL. Functional expression of CD28 on T cell antigen receptor γ/δ-bearing T lymphocytes. Eur J Immunol. 1989; 19:185-188. (Biology).

View All (8)

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

Analyte Specific Reagent. Analytical and performance characteristics are not established.