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BV421 Rat Anti-Mouse CD93 (Early B Lineage)
BV421 Rat Anti-Mouse CD93 (Early B Lineage)
Multiparameter flow cytometric analysis using BD OptiBuild™ BV421 Rat Anti-Mouse CD93 (Early B Lineage) antibody (Cat. No. 740064) on live C57/BL6 mouse bone marrow leukocytes. Flow cytometry was performed using a BD LSRFortessa™ X-20 Flow Cytometer System.
Multiparameter flow cytometric analysis using BD OptiBuild™ BV421 Rat Anti-Mouse CD93 (Early B Lineage) antibody (Cat. No. 740064) on live C57/BL6 mouse bone marrow leukocytes. Flow cytometry was performed using a BD LSRFortessa™ X-20 Flow Cytometer System.
Product Details
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BD OptiBuild™
Cd93; Ly68; C1qr1; C1qrp; Complement component C1q receptor
Mouse (Tested in Development)
Rat LEW, also known as Lewis IgG2a, κ
Pro-B cell line R2BFL derived from the fetal liver of a RAG-2-/-Bcl2-transgenic mouse
Flow cytometry (Qualified)
0.2 mg/ml
17064
AB_2739829
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV421 under optimal conditions that minimize unconjugated dye and antibody.

Recommended Assay Procedures

For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes (including BD Optibuild Brilliant reagents) are used in the same experiment.  Fluorescent dye interactions may cause staining artifacts which may affect data interpretation.  The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions.  More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794/566349) or the BD Horizon Brilliant Stain Buffer Plus (Cat. No. 566385).

Product Notices

  1. This antibody was developed for use in flow cytometry.
  2. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
  3. Researchers should determine the optimal concentration of this reagent for their individual applications.
  4. An isotype control should be used at the same concentration as the antibody of interest.
  5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  7. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
  8. BD Horizon Brilliant Violet 421 is covered by one or more of the following US patents: 8,158,444; 8,362,193; 8,575,303; 8,354,239.
  9. Pacific Blue™ is a trademark of Molecular Probes, Inc., Eugene, OR.
  10. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
740064 Rev. 3
Antibody Details
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493

The 493 antibody reacts with a cell-surface protein of 130-140 kDa expressed on immature B lymphocytes and a small fraction of newly formed B cells, but not on mature B lymphocytes. The antigen's distribution was defined through the use of antibodies to CD24 (Heat Stable Antigen), IgM, IgD, and CD45R/B220, which are commonly used to discriminate B-cell maturation stages.  493 mAb reacts with the majority of B220+ cells in bone marrow and a fraction of B220+ B cells in spleen, which are CD24[high], IgM[high], and IgD[low].  Cells binding 493 mAb were not detected in thymus, lymph nodes, or peritoneal cavity. This result suggested that 493 mAb does not stain B-1 B cells (CD5+ B lymphocytes), which are particularly found in the peritoneal cavity.  The 493 mAb does not seem to have any biological effect when incubated with immature B lymphocytes.  It has been observed that the staining pattern of mAb 493 is similar to that of mAb AA4.1, that both antibodies precipitate molecules of the same molecular weight, and that staining by mAb AA4.1 is not blocked by mAb 493, suggesting that the antibodies recognize separate epitopes of the same Early B Lineage antigen.

The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates.

740064 Rev. 3
Format Details
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BV421
The BD Horizon Brilliant Violet™ 421 (BV421) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye has an excitation maximum (Ex Max) of 407-nm and an emission maximum (Em Max) at 423-nm. Driven by BD innovation, BV421 is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 420-nm (e.g., a 431/28-nm or 450/50-nm bandpass filter). BV421 is an ideal alternative for V450 as it is approximately ten times brighter with less spillover into the BV510/V500 detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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BV421
Violet 405 nm
407 nm
423 nm
740064 Rev.3
Citations & References
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Development References (3)

  1. Allman D, Lindsley RC, DeMuth W, Rudd K, Shinton SA, Hardy RR. Resolution of three nonproliferative immature splenic B cell subsets reveals multiple selection points during peripheral B cell maturation. J Immunol. 2001; 167(12):6834-6840. (Biology). View Reference
  2. Rolink AG, Andersson J, Melchers F. Characterization of immature B cells by a novel monoclonal antibody, by turnover and by mitogen reactivity. Eur J Immunol. 1998; 28(11):3738-3748. (Immunogen). View Reference
  3. Rolink AG, Andersson J, Melchers F. Molecular mechanisms guiding late stages of B-cell development.. Immunol Rev. 2004; 197:41-50. (Clone-specific: Flow cytometry). View Reference
740064 Rev. 3

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.