Mouse IgG1 APC-R700

BD Horizon™ Mouse IgG1 APC-R700

Name: Mouse IgG1 APC-R700
Brand: BD Horizon™
SKU: 657709

Clone X40

(ASR)

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Product Details

Brand: BD Horizon™

Alternative Name: IgG1, kappa Isotype Control (Anti-KLH)

Isotype: Mouse BALB/c IgG1, κ

Immunogen: Keyhole limpet hemocyanin (KLH)

Application: Flow cytometry

Concentration: 100 µg/mL

Vol. Per Test: 5 μL

Storage Buffer: Phosphate buffered saline with BSA and ProClin.

Regulatory Status: ASR

Preparation And Storage

Store vials at 2°C–8°C. Conjugated forms should not be frozen. Protect from exposure to light. Each reagent is stable until the expiration date shown on the bottle label when stored as directed.

657709 Rev. 1

Antibody Details

X40

The Mouse IgG₁ antibody, clone X40, is derived from the hybridization of Sp2/0-Ag 14 mouse myeloma cells with spleen cells from BALB/c mice immunized with KLH.

The antibodies react specifically with keyhole limpet hemocyanin (KLH), an antigen not expressed on human cells or human cell lines.

657709 Rev. 1

Format Details

APC-R700

The BD Horizon™ APC-R700 (APC-R700) Dye is a part of the BD APC red family of dyes. This tandem fluorochrome is comprised of an Allophycocyanin (APC) dye donor that has excitation maximum (Ex Max) of 651-nm and an acceptor dye, R700, with an emission maximum (Em Max) at 706-nm. APC-R700, driven by BD innovation, is designed to be excited by the red (627–640-nm) laser and detected using an optical filter centered near 710-nm (e.g., a 720/40-nm bandpass filter). APC-R700 is a brighter alternative to Alexa Fluor™ 700. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: APC-R700

Excitation Source: Red 627-640 nm

Excitation Max: 651 nm

Emission Max: 706 nm

657709 Rev.1

Citations & References

Development References (2)

Centers for Disease Control. Update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in healthcare settings. MMWR. 1988; 37:377-388. (Biology).
Clinical and Laboratory Standards Institute. 2005. (Biology).

657709 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

Analyte Specific Reagent. Analytical and performance characteristics are not established.