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      Purified Mouse Anti-Stat4 (pY693)
      Purified Mouse Anti-Stat4 (pY693)
      K-562 cells were either left untreated (lane 1) or treated (lane 2) with 1.0 mM pervanadate for 20 minutes at 37°C. The image shows a blot that was probed with Stat4 (pY693).
      Purified Mouse Anti-Stat4 (pY693)
      K-562 cells were either left untreated (lane 1) or treated (lane 2) with 1.0 mM pervanadate for 20 minutes at 37°C. The image shows a blot that was probed with Stat4 (pY693).
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      BD Transduction Laboratories™
      Human (QC Testing), Mouse (Tested in Development)
      Mouse IgG2b, κ
      Phosphorylated Human Stat4 (pY693)
      Western blot (Routinely Tested)
      89 kDa
      250 µg/ml
      AB_399958
      Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.
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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
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      612738 Rev. 1
      Antibody Details
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      38/p-Stat4

      The Stat proteins function both as cytoplasmic signal transducers and as activators of transcription. Seven mammalian Stat proteins have been identified: Stat1-4, Stat5a, 5b, and Stat6.  Stat4 has been shown to play an important role in development of T helper cells, specifically the Th1 subset. Stat4 is activated by IL-12 and by type 1 interferons. Knockout mice supported the role that Stat4 plays in IL-12 signaling because lymphocytes from Stat 4-/- mice could neither differentiate into Th1 cells nor produce IFN-γ in response to treatment with IL-12. IFN-γ plays an important role in host defense. A key component in the activation of Stat4 is the phosphorylation on tyrosine and serine residues in response to IL-12 stimulation. IL-12 stimulation leads to the phosphorylation of Stat4 on tyrosine 693 and serine 721. Transcriptional activity of Stat4 has been shown to be significantly reduced when residues Y693 and S721 are mutated.

      612738 Rev. 1
      Format Details
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      Purified
      Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
      Purified
      612738 Rev.1
      Citations & References
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      Development References (2)

      1. Kisseleva T, Bhattacharya S, Braunstein J, Schindler CW. Signaling through the JAK/STAT pathway, recent advances and future challenges. Gene. 2002; 285:1-24. (Biology).
      2. Visconti R, Gadina M, Chiariello M, et al. Importance of the MKK6/p38 pathway for interleukin-12−induced STAT4 serine phosphorylation and transcriptional activity. Blood. 2000; 96:1844-1852. (Biology).
      612738 Rev. 1

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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