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PE Rat Anti-Mouse PDC-TREM
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PE Rat Anti-Mouse PDC-TREM
Multi-color flow cytometric analysis of PDC-TREM expression on mouse plasmacytoid dentritic cells (pDCs). Splenocytes from BALB/c mice were cultured in the presence of CpG-A (Invivogen) for 24 hours. The cells were then washed, and Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™) (Cat. No. 553141 or 553142) was added. The cells were stained simultaneously with optimal concentrations of FITC Hamster Anti-Mouse CD3e (Cat. No. 553061, 553062, or 561827), FITC Rat anti-Mouse CD19 (Cat. No. 553785, 557398, or 561740), APC Rat Anti-Mouse CD45R/B220 (Cat. No. 553092 or 561880), and BD Horizon™ BUV395 Hamster Anti-Mouse CD11c (Cat. No. 564080), plus either PE Rat IgG2a, κ Isotype Control (Cat.No. 553930; dashed line histogram) or PE Rat Anti-Mouse PDC-TREM (Cat. No. 566926; solid line histogram) at 0.5 µg/test. The left plot was derived from CD3e-negative, CD19-negative viable leukocytes and displays the gating of the CD45R/B220-positive, CD11c-positive pDC population. The right plot shows the expression of PDC-TREM (or Ig Isotype Control staining) on the gated pDCs. Flow cytometry and data analysis was performed using a BD LSRFortessa™ Flow Cytometer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.
Multi-color flow cytometric analysis of PDC-TREM expression on mouse plasmacytoid dentritic cells (pDCs). Splenocytes from BALB/c mice were cultured in the presence of CpG-A (Invivogen) for 24 hours. The cells were then washed, and Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™) (Cat. No. 553141 or 553142) was added. The cells were stained simultaneously with optimal concentrations of FITC Hamster Anti-Mouse CD3e (Cat. No. 553061, 553062, or 561827), FITC Rat anti-Mouse CD19 (Cat. No. 553785, 557398, or 561740), APC Rat Anti-Mouse CD45R/B220 (Cat. No. 553092 or 561880), and BD Horizon™ BUV395 Hamster Anti-Mouse CD11c (Cat. No. 564080), plus either PE Rat IgG2a, κ Isotype Control (Cat.No. 553930; dashed line histogram) or PE Rat Anti-Mouse PDC-TREM (Cat. No. 566926; solid line histogram) at 0.5 µg/test. The left plot was derived from CD3e-negative, CD19-negative viable leukocytes and displays the gating of the CD45R/B220-positive, CD11c-positive pDC population. The right plot shows the expression of PDC-TREM (or Ig Isotype Control staining) on the gated pDCs. Flow cytometry and data analysis was performed using a BD LSRFortessa™ Flow Cytometer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.
Product Details
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BD Pharmingen™
A530064D06Rik; pdctrem; plasmacytoid dendritic cell-specific receptor; Trem4; triggering receptor expressed on myeloid cells 4
Mouse (QC Testing)
Rat WI, also known as Wistar (outbred) IgG2a, κ
Mouse PDC-TREM Recombinant Protein
Flow cytometry (Routinely Tested)
0.2 mg/ml
AB_2869951
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Recommended Assay Procedures

BD™ CompBeads can be used as surrogates to assess fluorescence spillover (Compensation).  When fluorochrome conjugated antibodies are bound to CompBeads, they have spectral properties very similar to cells.   However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls.  It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and CompBead to ensure that BD Comp beads are appropriate for your specific cellular application.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  5. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
  6. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
566926 Rev. 1
Antibody Details
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4A6

The 4A6 monoclonal antibody specifically recognizes mouse PDC-TREM, also known as TREM-4, which is a member of the Triggering Receptor Expressed on Myeloid cells (TREM) family of protein-binding receptors that regulate innate immune responses. Stimulation of plasmacytoid dendritic cells (pDCs) through toll-like receptors (TLRs) induces type I interferon production and PDC-TREM expression. Cell-surface expression and immune regulatory signaling by PDC-TREM require its association with Plexin-A1 and DNAX-activation Protein 12 (DAP12) and further augments type I interferon production by pDCs.

566926 Rev. 1
Format Details
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PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
566926 Rev.1
Citations & References
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Development References (5)

  1. Kasamatsu J, Deng M, Azuma M, et al. Double-stranded RNA analog and type I interferon regulate expression of Trem paired receptors in murine myeloid cells. BMC Immunol. 2016; 17(1):9. (Biology). View Reference
  2. Rahim MM, Tai LH, Troke AD, et al. Ly49Q positively regulates type I IFN production by plasmacytoid dendritic cells in an immunoreceptor tyrosine-based inhibitory motif-dependent manner.. J Immunol. 2013; 190(8):3994-4004. (Clone-specific: Flow cytometry). View Reference
  3. Roe K, Gibot S, Verma S. Triggering receptor expressed on myeloid cells-1 (TREM-1): a new player in antiviral immunity?. Front Microbiol. 2014; 5:627. (Biology). View Reference
  4. Swiecki M, Wang Y, Vermi W, Gilfillan S, Schreiber RD, Colonna M. Type I interferon negatively controls plasmacytoid dendritic cell numbers in vivo. J Exp Med. 2011; 208(12):2367-2374. (Biology). View Reference
  5. Watarai H, Sekine E, Inoue S, Nakagawa R, Kaisho T, Taniguchi M. PDC-TREM, a plasmacytoid dendritic cell-specific receptor, is responsible for augmented production of type I interferon.. Proc Natl Acad Sci USA. 2008; 105(8):2993-8. (Immunogen: Flow cytometry). View Reference
View All (5) View Less
566926 Rev. 1

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.