PE Mouse Anti-Human MSR1 (CD204)

Consider BD Horizon RealYellow™ 586 (RY586) Reagents, a bright and clean fluorochrome alternative to PE off the yellow-green laser. RY586 can be used alongside PE on spectral cytometers. More Info

BD Pharmingen™ PE Mouse Anti-Human MSR1 (CD204)

Name: PE Mouse Anti-Human MSR1 (CD204)
Brand: BD Pharmingen™
SKU: 566251

Clone U23-56

(RUO)

Flow cytometric analysis of MSR1 (CD204) expression on human monocyte-derived macrophages. Freshly isolated human monocytes were cultured (6 days) with Recombinant Human GM-CSF protein (Cat. No. 550068; 50 ng/ml). The cells were harvested with an enzyme-free cell dissociation solution, washed, and then stained with either PE Mouse IgG1, κ Isotype Control (Cat. No. 554680; dashed line histogram) or PE Mouse Anti-Human MSR1 (CD204) antibody (Cat. No. 566251; solid histogram). The histogram showing MSR1 (CD204) expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of viable macrophages. Flow cytometric analysis was performed using a BD™ Canto II Flow Cytometry System.

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Product Details

Brand: BD Pharmingen™

Alternative Name: MSR; MSRE; phSR1; phSR2; SCARA1; SR-A; SRA

Reactivity: Human (QC Testing), Mouse (Lack of Reactivity Confirmed in Development)

Isotype: Mouse IgG1, κ

Immunogen: Human MSR1 Recombinant Protein

Application: Flow cytometry (Routinely Tested)

Concentration: 0.2 mg/ml

RRID: AB_2739630

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

Product Notices

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
An isotype control should be used at the same concentration as the antibody of interest.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.

Companion Products

Stain Buffer (FBS) RUO

Size 500 mL Cat No. 554656

Stain Buffer (BSA) RUO

Size 500 mL Cat No. 554657

PE Mouse IgG1, κ Isotype Control RUO

Size 0.1 mg Cat No. 554680

Recombinant Human GM-CSF RUO

Size 10 µg Cat No. 550068

566251 Rev. 1

Antibody Details

U23-56

The U23-56 monoclonal antibody specifically binds to Macrophage scavenger receptor 1 (MSR1) which is also known as CD204, Scavenger receptor A (SRA), Scavenger receptor class A member 1 (SCARA1), or Macrophage acetylated LDL receptor I and II. MSR1 is a trimeric type II transmembrane glycoprotein that belongs to the type 1 class A scavenger receptor family. MSR1 functions as a scavenger receptor that enables mononuclear phagocytes to take up and respond to a variety of negatively charged macromolecules including oxidized and acetylated low density lipoproteins. MSR1 also functions in innate immunity as a pattern recognition receptor. MSR1 allows macrophages to recognize and respond to microbial products, such as lipopolysaccharide and polyribonucleic acids, by the production of cytokines and inflammatory mediators. MSR1 also reportedly serves as a coreceptor that regulates the signaling of some Toll-like receptors including TLR-3, TLR-4, and TLR-9. MSR1 has been associated with several diseases including atherosclerosis, Alzheimer's disease, ischemic injury, and sepsis. The U23-56 antibody was tested by flow cytometric and Western blot analyses of MSR1-transfected and untransfected cells to verify its specificity.

566251 Rev. 1

Format Details

R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: PE

Excitation Source: Yellow-Green 488 nm, 532 nm, 561 nm

Excitation Max: 496 nm, 566 nm

Emission Max: 576 nm

566251 Rev.1

Citations & References

Development References (4)

Gough PJ, Greaves DR, Gordon S. A naturally occurring isoform of the human macrophage scavenger receptor (SR-A) gene generated by alternative splicing blocks modified LDL uptake.. J Lipid Res. 1998; 39(3):531-43. (Biology). View Reference
Higashi-Kuwata N, Jinnin M, Makino T, et al. Characterization of monocyte/macrophage subsets in the skin and peripheral blood derived from patients with systemic sclerosis.. Arthritis Res Ther. 2010; 12(4):R128. (Biology). View Reference
Hongo S, Watanabe T, Arita S, et al. Leptin modulates ACAT1 expression and cholesterol efflux from human macrophages.. Am J Physiol Endocrinol Metab. 2009; 297(2):E474-82. (Biology). View Reference
Zola H, Swart B, Nicholson I, Voss E. CD204. In: Zola H. Leukocyte and stromal cell molecules : the CD markers. Hoboken, N.J.: Wiley-Liss; 2007:347-348.

View All (4)

566251 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.